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Baculovirus expression system

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Since its inception in 1983, baculovirus expression vector system (BEVS) has been widely used in the preparation of various recombinant proteins, especially in the expression of recombinant kinases and protein complexes.


Researchers of Medicilon established a well-developed baculovirus-insect cell expression service platform, which offers recombinant baculovirus preparation, recombinant protein and its compound expression and purification services.

Baculovirus Expression Vector System

Baculovirus expression vector system (BEVS) is a power system to produce a variety of prokaryotic and eukaryotic protein.  It is also an efficient tool for preparing vaccines.

The system could effectively produce high yield target protein with proper folding and post-translation modifications in an intracellular or secretion expression means.

Medicilon researchers establish a well-developed baculovirus-insect cell expression services platform. We provide the expression and purification services on preparation of recombinant baculovirus and recombinant protein. We have a good track record in producing kinase and recombinant protein complexes.

Service:

  1. Generation of recombinant Bacmid DNA
  2. Preparation of recombinant baculovirus
  3. Titration of baculovirus
  4. Protein expression verification and optimization
  5. Small scale expression and purification of recombinant protein in insect cell
  6. Scale up expression and purification of recombinant protein in insect cell
Steps Items Timeline Comments
1 Generation of recombinant Bacmid DNA 3 weeks Different affinity tag: GST tag, His tag, Flag tag…..
2 Preparation of recombinant baculovirus 2 weeks
3 Titration of baculovirus 2-3 days Quantitative-PCR or based on gp64 antibody
4 Protein expression verification and optimization 2 weeks MOIs, PIs or different cell lines
5 Small scale expression and purification 2-4 weeks 500 ml-2L expression in flask
6 Scale up expression and purification TBD Culture in flask or fermentor

Remarks:The service is highly flexible to meet your request. One or more above steps could be separately provided.


Advantages of Baculovirus Expression

  • Fast growth
  • Cheap medium and equipment for growing
  • Good knowledge of the host


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