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E.coli protein expression system

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E. coli expression system is the earliest developed and most widely used gene expression system. In recent decades, E.coli expression system has been popularly used and developed for producing a wide variety of different types of proteins. Compared with other expression systems, E. coli is a well-established host with the advantages of high gene expression level, short cultivation time, strong anti-polluted ability and relatively low cost.


Researchers of Medicilon established a well-developed E. Coli Expression and Purification Services Platform, which offers the expression and purification services of a variety of recombinant proteins.

E. coli Expression System

E. coli expression system is the earliest developed and most widely used gene expression system. In recent decades, E.coli expression system has been popularly used and developed for producing a wide variety of different types of proteins. Compared with other expression systems, E. coli is a well-established host with the advantages of short culturing time, easy genetic manipulation and low cost media.

Medicilon researchers established a well-developed E. coli expression and purification services platform, which offers the expression and purification services of a variety of recombinant proteins, with a fast turn-around time and competitive pricing.

Service:

  1. Generation of Recombinant Plasmid
  2. Expression Test in Host
  3. Soluble Expression Test
  4. Small Scale Expression and Purification (2L Cell Culture)
  5. Scale Up Expression and Purification
Steps Items Timeline Comments
1 Generation of Recombinant Plasmid 2 weeks Multiple expression vector, different affinity tag: His, Trx , GST, SUMO…
2 Expression of Recombinant Protein 1 week Multiple expression host
3 Soluble Expression Optimization 1-2 weeks Test different induction concentration, time point, temperatures or reconstruct plasmids
4 Small Scale Expression and Purification 1-2 weeks Purification of soluble expression or from inclusion body
5 Scale Up Expression and Purification TBD Culture in flask or fermenter

Remarks:

  1. The service is highly flexible to meet your request. One or more above steps could be separately provided.
  2. Affinity purification, ion exchange purification, hydrophobic beads purification and size exclusion column purification or their combination will be used for protein purification.



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