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Yeast expression system

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Yeast Expression System has the advantages of both prokaryotic and higher eukaryotic systems, with the characteristics of common culture conditions, fast growth rate, relatively low cost, processed after protein translation, easy to obtain soluble active recombinant protein, is widely used in recombinant protein, especially the preparation of eukaryotic proteins. Because of its fast growth, rich commercial expression vector and highly efficient secretion of expression, Pichia pastoris becomes the priority for yeast expression of recombinant protein.


Researchers of Medicilon established a well-developed Yeast Expression System and Purification Services Platform, which offers the expression and purification of high quality recombinant proteins in Pichia pastoris and saccharomyces cerevisiae.

Yeast Expression System

Yeast expression system contains the advantages of both prokaryotic and eukaryotic system, such as easy scaling up, high expression level, proper protein folding, fast growth rate and low cost, which makes them widely used in industry and research field.

Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris are commonly used expression strain. Pichia pastoris is more popular, especially in the large-scale production of eukaryotic recombinant proteins, due to the fast growth rate, multiple commercial expression vectors and high efficiency of secretion expression.

Medicilon provides a well-developed yeast expression and purification services, including the mini-scale and large scale expression and purification of recombinant protein in Pichia pastoris and Saccharomyces cerevisiae.

Service:

  1. Recombinant Plasmid Generation for Yeast Expression
  2. Transformation of Linear Construct
  3. Verification of Positive Transformants by PCR and Western Blot Method
  4. Small Scale Expression and Purification (2L cell culture)
  5. Scale Up Expression and Purification of Recombinant Protein (10L or more)
Steps Items Timeline
1 Recombinant Plasmid Generation 2 weeks
2 Transformation of Linear Construct 1 week
3 Verification of Positive Transformants via PCR 3-4weeks
4 Small Scale Expression and Purification 2-4 weeks
5 Scale Up Expression and Purification TBD

Remarks:

  1. The service is highly flexible to meet your request. One or more above steps could be separately provided.
  2. Affinity purification, ion exchange purification, hydrophobic beads purification and size exclusion column purification or their combination will be used for protein purification.


Yeast expression system contains the advantages of both prokaryotic and eukaryotic system, such as easy scaling up, high expression level, proper protein folding, fast growth rate and low cost, which makes them widely used in industry and research field.

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