China CRO Company,Preclinical research services

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Crystallization & Structural Determination

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Medicilon established a well-developed structural biology research platform.  Medicilon is proud as the first CRO to provide gene to crystal structure service in China.  We have well-established integrated service platform, which has been certified as an important platform for drug discovery by Shanghai Government.

Protein Crystallization & Structural

Medicilon established a well-developed structural biology research platform.  Medicilon is proud as the first CRO to provide gene to crystal structure service in China.  We have well-established integrated service platform, which has been certified as an important platform for drug discovery by Shanghai Government.

Medicilon has more than ten years of experiences in providing gene to crystal structure service, including construction design, protein expression and purification, high-throughput screen of crystallization conditions, compound or fragments soaking and structure determination.

Service: 

  1. Crystallization condition screen by Mosquito
  2. X-ray diffraction data collection at Synchrone
  3. Co-crystallization of Protein-compound; Fragment screen by soaking or co-crystallization
  4. Determination of structure
Steps Items Timeline Comments
1 Crystallization condition screen by Mosquito 4-8 weeks Commercial screen kits, Sparse matrix screen, 18 oC and 4 oC
2 X-ray diffraction data collection at Synchrone 4-8 weeks Collected at SSRF weekly
3 A. Co-crystallization of Protein-compound
B. Fragment Screen by soaking or co-crystallization
4-8 weeks Binding affinity date of compounds with protein is crucial
4 Determination of structure TBD Phase solved by MR, SeMet or MIR

Remarks: The service is highly flexible to meet your request. One or more above steps could be separately provided.

Various Crystals Grown At Medicilon

   
Build the homology model of PI3K delta   Compared 3D structures of PI3K isoforms, identify critical residues and pocket features for selectivity and affinity   Compounds were designed based on protein structures

Research papers

X‑ray Crystal Structure of Phosphodiesterase 2 in Complex with a Highly Selective, Nanomolar Inhibitor Reveals a Binding-Induced Pocket Important for Selectivity
Thymoquinone Blocks pSer/pThr Recognition by Plk1 Polo-Box Domain As a Phosphate Mimic

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