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Double immunofluorescence When detecting two antigens at the same time in the same tissue or cell sample, double fluorescent staining is required. Double immunofluorescence labeling method (double immunofluorescence labeling method) is also divided into direct method and indirect method. Direct double immunofluorescence labeling Direct double immunofluorescence labeling: mix two different fluorescein-labeled antibodies (such as anti-ARead more

Immunofluorescence technology combines fluorescein such as fluorescein isothiocyanate (FITC) with the corresponding antibody (or antigen) in a chemical way, and combines the fluorescent-labeled antibody (or antigen) with the corresponding antigen in the specimen to form a fluorescent-labeled antibody -Antigen complex, observe with a fluorescence microscope. The presence of fluorescence under the microscope indicates the presenceRead more

Autoimmune disease refers to a series of pathological immune responses produced by the body’s immune effect or immune effector molecules against one’s own tissues or cells, leading to a large class of diseases that damage their own tissues and organs. The pathogenesis is very complex and the clinical manifestations are variable. , Often involving theRead more

Fluorescence immunoassay technology is one of the earliest developed labeling immunoassay techniques. For a long time, some scholars have tried to combine antibody molecules with some tracer substances, and use antigen-antibody reaction to locate antigenic substances in tissues or cells. Coons was equal to the first successful use of fluorescein for labeling in 1941. ThisRead more

The dissolution rate and extent of solid preparations such as tablets, capsules, or granules in a prescribed medium refer to the dissolution rate of the drug. Since the dissolution of the drug will directly affect the absorption and utilization of the drug in the body, the drug dissolution test is an important indicator to evaluateRead more

The application of antibodies has a history of hundreds of years. As a disease prevention, diagnosis and treatment agent, antibody drugs have undergone the development of murine antibodies, chimeric antibodies, humanized antibodies and fully humanized antibodies. Because the antibody drug is a kind of foreign protein, it is easy to activate the body’s own immuneRead more

Immunogenicity refers to the nature of drugs that stimulate the body to form specific antibodies or sensitized lymphocytes. In the development of biotechnology drugs, immunogenicity testing is an important task. For patients, immunogenicity will affect the safety and effectiveness of drugs, and even cause life-threatening dangers to patients because of the crossover of anti-drug antibodiesRead more

Recently, cancer researchers at Vanderbilt discovered a new protein that, when genetically regulated to prevent it from interacting with the genes responsible for cancer, will effectively melt tumors within a few days. This exciting research result was recently published in eLife magazine. protein that can melt tumors You have never heard of the protein thatRead more

Lung cancer is one of the most harmful malignant tumors. To explore the pathogenesis of lung cancer and to find new targets for anti-tumor therapy are the hotspots and difficulties of current lung cancer treatment research. Studies have found that aquaporin 4 (AQP4) is highly expressed in lung cancer cells, and knocking down AQP4 canRead more

Cytokines that can regulate the physiological functions of a variety of cells are small molecular protein substances with biological activity secreted by cells. The concentration of cytokines and their receptors can reflect the pathological process of the disease. Through the detection of cytokines and the detection of the level of soluble receptors, the effects ofRead more