Medicilon provides the following types of Gene-to-Protein services:

Cloning
·Plasmid construction and modification
·Site-directed mutagenesis

Cell Culture
·Bacterial, Pichia pastoris, mammalian, and baculoviral expression systems
·Culturing, transient and stable transfection of mammalian cell lines
·Production and amplification of recombinant baculoviruses in insect cells (Sf9, Sf21,High Five)
·Process development in baculovirus expression
·Scale-up of bacterial, Pichia pastoris, insect-cell, and mammalian cultures (up to 20-L)

Protein Purification (up to several hundreds mg)
·Ion-exchange chromatography
·Size exclusion chromatography
·Affinity chromatography
·Hydrophobic interaction chromatography

Quality Control
·Activity validation
·QC by N-terminal sequencing, Western blot,SDS PAGE, and mass spectrometry