M9 Sales

Structural Medicilon offers prepackaged M9 selenomethionine (SeMET) growth media kits and packages for growing cell culture in IPTG-inducible bacterial expression systems leading to the production of SeMET-labeled proteins for crystallographic studies using MAD. The cell growth media kits are designed to simplify the procedures to grow cell culture and save your time and money. We have optimized the kits (see below) for structural genomics laboratories to speed up the protein structure production; we can also make kits specially designed for you. These kits eliminate the need for sterilization and have no need for weighing out chemicals; just add kits one by one and have cells grown in the end. The high-yield media kits have been optimized to double the cell yield and have become popular.

M9 SeMET Growth Media Kit Packages

Packages	Catalog #	Price ($)	Unit Price ($) per L
M9 SeMET growth media, packaged sufficient for growing liter culture. kit A, kit B, kit C, kit D and kit E for 12-L	MD045003-12L	198/set	16.5
M9 SeMET growth media, packaged for growing 50 X1 liter MD045003-50L culture. kit A, kit B, kit C, kit D and kit E for 50-L	MD045003-50L	800/set	16
M9 SeMET high-yield media, packaged for growing 12X1 liter culture. kit A(H), kit B(H), kit C(H), kit D and kit E for 12-L	MD045004-12L	360/set	30
M9 SeMET high-yield media, packaged for growing 50X1 liter culture. kit A(H), kit B(H), kit C(H), kit D and kit E for 50-L MD045004-50L	MD045004-50L	1500/set	30

Components of Media Kits are available separately as listed in the following:
L-(+)-Selenomethionine (SeMET) $ 99/g
CAS#: [3211-76-5]; analytical grade

	Catalog #	Qty ($)	Price ($)
((S)-2-Amino-4-(methylseleno)butyric acid C5H11NO2Se FW 196.1 minimum 98% (TLC), Powder Color .....................................white to off-white Solubility (water) ............................... 50 mg/ml Use: In the M9 SeMET growth media kits, SeMet is mixed with the inhibitory 6 amino acids, prepackaged for growing 12X1 liters of culture.	MD045003D MD045003D MD045003D MD045003D	100mg 1g 10g 12L	30 99 900 92

SeMET GROWTH MEDIA KITS

M9 SeMET growth media kits $ 16/liter culture (SeMET included)
Catalog number: MD045003
SeMET-labeled protein production for crystallographic studies using MAD phasing No need for autoclave/sterilization nor for weighing out; just add Kits to cell solutions and done! All are cell-tested. We would custom-made and pre-package the kits to meet your special needs.
Procedure for growing 1 liter culture in a 2-L container

1) Add one pouch of Kit A and then add ~980 ml of water
2) Add 1 ml of Kit B solution
3) Add 1 ml of Kit C solution
4) Add 10 ml of the inoculated culture and shake at 37 oC till OD 0.8~1.0
5) Add 20 ml kit D solution and lower the temperature to 20℃
6) 15 minutes later, add 1 ml of Kit E solution
7) Growing cell at 20 oC overnight

M9 SeMET growth media kits $ 16/liter culture (SeMET included)

Catalog number: MD045004	Catalog #	Price
M9 Kit A ............................................................. Na/K/PO4, C/N sources............................1 liter culture M9 Kit B (Mineral supplement) ................ One pouch to make 12ml solution ........12 X1 liter culture One pouch to make 50 ml solution........50 X1 liter culture M9 Kit C (Antibiotics/Vitamin)................ One pouch to make 12ml solution ........12 X1 liter culture One pouch to make 50 ml solution........50 X1 liter culture need 1 ml for 1 liter of cell......................... M9 Kit D (Inhibitory Amino Acids plus SeMET).. One pouch to make 250 ml solution........12 X1 liter culture One pouch to make 1000 mL solution....50 X1 liter culture need 20 ml for 1 liter of cell culture................. M9 Kit E (IPTG).......................................... One pouch to make 12ml solution ........12 X1 liter culture One pouch to make 50 ml solution........50 X1 liter culture need 1 ml for 1 liter of culture.................	MD045003A MD045003B -12L -50L MD045003C -12L -50L MD045003D -12L -50L MD045003E -12L -50L	$6.7/pouch $2.5/pouch $3.5/pouch $7/pouch $21/pouch $92/pouch $384/pouch $19/pouch $74/pouch

M9 SeMET High-yield Growth Media Kits

$ 30/Liter Culture (SeMET included)

Catalog number: MD045004	Catalog #	Price
M9 Kit A(H)...................... Na/K/PO4, C/N sources, noninhibitory amino acids ..1 liter culture M9 Kit B(H) (Mineral supplement)................. ........................................................25 liter culture M9 Kit C(H)....................... Antibiotics/ Vitamins to make 12 ml solution.....12 X 1 liter culture Antibiotics/ Vitamins to make 50 ml solution.....50 X 1 liter culture need 1 ml for 1 liter of cell............... M9 Kit D.......................................... One pouch to make 250 ml solution.........12 X1 liter culture One pouch to make 1000 mL solution.....50 X1 liter culture need 20 ml for 1 liter of cell culture........... M9 Kit E ......................................................... IPTG -One pouch to make 12 ml solution....12 X1 liter culture One pouch to make 50 ml solution...............50 X1 liter culture Need 1 ml for 1 liter of culture..........................	MD045004A MD045004B MD045004C -12L -50L MD045003D -12L -50L MD045003E -12L -50L	$12/pouch $223/250ml $15/pouch $33/pouch $92/pouch $384/pouch $19/pouch $74/pouch

ORDER INFORMATION

Please order:
Phone: +1 847 596 0856 ; +86 21 51320497 *228
Fax: +1 847 298 5008 ; +86 21 51320497 *220
E-mail: crystal@medicilon.com.cn
Please provide the following information: item name or catalog #, amount, descriptions, shipping address; if use credit card (Visa, Master, Discover accepted), provide credit card number and expiration date; otherwise provide billing address. All ready to ship, simple.
(For ordering growth media, please indicate what antibiotics that you use).
The typical order is like the following:
Catalog # Qty Unit Price Total Description
MD045003-12L $198 M9 SeMET growth media kit package for 12X1 L
MD045004-12L $360 M9 SeMET high yield growth media kit package for 12X1 L
MD45003D-10g $990 L-(+)-Selenomethionine

*Note:
1) Since the grow media kits include SeMET, there is no need to order SeMET separately;
please also indicate what antibiotics you want us to prepare in the growth media == ampicilin or kanamycin or chloramphenicol etc. or add-ityourself? would be respected.
2) Credit card # /expiration date or PO# xxxx xxxx xxxx xxxx / exp. xx/xx
3) or billing address
4) Shipping address

SERVICES

We have most of the chemicals/reagents in stock, routinely used for HT structural biology programs. Let us know how you like your package to be made without extra cost and see how we can assist you the best. The product will be delivered with complete instructions, within a week, unless special packaging is requested. Add two days for Custom clearance for deliveries outside the continent.
Contact us for a catalog of other chemicals used in structural biology: mail to crystal@medicilon.com.cn

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