Molecular imprinting technology derived from immunology is a technology that can be used for drug analysis and detection. The material preparation technology for obtaining molecularly imprinted polymers that match the spatial shape, size and functional group of a specific target molecule has been It is widely used in the fields of chromatographic separation, molecular recognition, biosensing and catalysis.
The template molecule used in the molecular imprinting technology is the target molecule that needs to be separated. The target molecule and the crosslinking agent are polymerized in a polymer monomer solution to obtain a monomer-template molecule complex, which is then removed by physical or chemical means The template molecule is used to obtain a molecularly imprinted polymer (MIP) with the spatial structure of the target molecule. Based on the high selectivity and better adsorption characteristics of molecularly imprinted polymers, it has been widely used in the identification, enrichment, separation, and drug analysis and detection of the drug to be tested. The molecular imprinting technology can be used for quantitative detection of various drugs. Medicilon provides drug analysis and testing services, including method development and method verification, analytical testing and release, stability studies, etc., which can be used for APIs and pharmaceutical preparations (tablets, capsules/liquid capsules, liquid preparations/emulsions, powder preparations, Pills/coated pills, injections, topical medications, solid dispersions, etc.) provide analysis and detection methods for stability studies and/or related substances.
In drug analysis and detection, molecular imprinting technology can be used to determine the active ingredients in drugs. For example, the chemical ingredients in traditional Chinese medicine are very complicated. After the active ingredients are determined, they can be used as template molecules to synthesize MIP, with holes similar in configuration to the template molecule. The corresponding action sites will remain on the MIPs, resulting in specific adsorption properties, and components similar to the template molecular structure will also remain in the MIPs. Some researchers have also established a method to determine the content of Clenbuterol in Chuanxiling by molecularly imprinted solid phase extraction-high performance liquid chromatography. Some researchers have used o-aminophenol as a polymerized monomer to obtain a mitoxantrone molecularly imprinted electrochemical sensor, which is used for the determination of mitoxantrone in drug analysis, and has high selectivity and Sensitivity.
As an analytical detection technology, many research groups have successively used molecular imprinting technology to quantitatively detect various drugs. Some researchers have established a molecular imprinting-chemiluminescence method to detect chlorpromazine hydrochloride, which is used as a substance recognized by molecular imprinting. This method has strong selectivity and high sensitivity.
Compared with other biological macromolecules such as enzymes and substrates, antigens and antibodies, receptors and hormones, molecularly imprinted polymers have the following advantages:
(1) The imprinting sites of MIPs are artificially synthesized and have strong physical and chemical resistance to environmental changes;
(2) The functional groups of MIPs can be easily modified to improve its affinity and catalytic ability;
(3) MIPs can be used multiple times without worrying about the loss of affinity;
(4) MIPs have strong resistance to harsh environments (such as solvents, ions, heat, acid and alkali damage);
(5) The preparation of MIPs does not require immunization of animals, but the obtained products have uniform properties and affinity, and the entire preparation process takes a very short time. Different molecularly imprinted polymers are prepared according to different template molecules, which can meet different needs in daily separation and analysis (such as food safety, drug separation, etc.).
Molecularly imprinted polymer is an effective method for drug separation and drug analysis and detection. It has a good application prospect in pharmacy. The highly selective molecularly imprinted separation material with the dual recognition ability of three-dimensional configuration and interaction site can not only be pure It can also separate mixtures with similar structures, and achieve rapid, accurate and efficient determination in drug pharmacology research and drug quality control. It is believed that the application of molecular imprinting technology in the field of pharmacy will become more and more extensive in the future .