Medicilon’s proteomics technology service integrates multiple technologies such as biochemistry, cell biology, molecular biology, and mass spectrometry to enable protein identification, differential protein quantitative analysis, complex separation, and protein post-translational modification (eg, phosphorylation) , Glycosylation, ubiquitination, etc.) analysis and proteomics informatics analysis. Proteomics essentially refers to studying the characteristics of proteins at a large scale, including protein expression levels, post-translational modifications, protein-protein interactions, etc., thereby obtaining information about disease occurrence, cell metabolism, etc. at the protein level A holistic and comprehensive understanding of the process.
The development of proteomics technology has become an important support for the rapid development of modern biotechnology, and will lead biotechnology to achieve a key breakthrough. To help workers in the life sciences field fully grasp the proteomics technology and methods, experimental difficulties and key points, research frontiers and hot spots, this technology platform will provide customers with proteomics technical services, including two-dimensional gel electrophoresis, isoelectric Focusing, electrospray mass spectrometry (ESI-MS), biological mass spectrometry and non-gel techniques.
Proteomics research techniques:
Sample preparation: Generally, all protein components in cells or tissues can be used for proteome analysis.
Sample separation and analysis: It is a very effective method to distinguish various proteins by two-dimensional gel electrophoresis using the isoelectric point and molecular weight of the protein.
New technologies for proteome research
- Protein identification: One-dimensional electrophoresis and two-dimensional electrophoresis combined with Western and other technologies are used to identify and study proteins using protein chip and antibody chip and co-immunoprecipitation technology.
- Post-translational modifications: Many proteins produced by mRNA expression undergo post-translational modifications such as phosphorylation, glycosylation, and zymogen activation.
- Protein function determination: such as analysis of enzyme activity and determination of enzyme substrates, biological analysis of cytokines/ligand-receptor binding analysis. Gene knockout and antisense techniques can be used to analyze the function of gene expression products-proteins.
- Find target molecules of drugs.