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Discovery Biology Services

Home Service Discovery Biology Services

Our Discovery Biology department offers services supporting structure-based drug discovery from determination of novel targets to final structures. Our platform is one of the earliest established structural biology platforms in China and has been certified by the Shanghai Government.

Biochemical Assay

Technology Platforms

  • Fluorescence: Lance; Alphascreen; Z’-LYTE ; HTRF,TR-FRET,FP etc
  • Luminescence: Kinase-Glo; ADP-Glo etc
  • Absorbance: UV; VI

Kinase Assay: VEGFR2 I

Protease Assay: Renin inhibition using FRET

Validated Biochemical Assay List

Validated Enzymatic Targets

Abl

AKT1

AKT2

AKT3

ALK

Aurora A

Aurora B

B-Raf

BTK

Cathepsin K/L/B/S

CDK/P25

CHK1

CHK2

c-Kit

c-Met

COX-1/COX-2

C-Raf

DPPIV

EGFR

EGFR (L858R/T790M)

Factor Xa

FGFR1

Flt1/VEGFR1

Flt3

Flt4

α- Glucosidase

β- Glucosidase

GSK3 beta

H+/K+ ATPase

HDAC1 &HDACs

HER2

IKK beta

JAK1

JAK2

JAK3

LCK

Lipoxygenase

Lyn

MAPK14

MARK1

MEK1

MEK2

mTOR

PDE5

PDGFR beta

PI3K alpha

PI3K beta

PI3K delta

PI3K gamma

PKA

PKC delta

PKC gamma

Plk1

20S proteasome

Renin

SAHase

SGK1

SRC

Syk

VEGFR2

Note: The enzyme in blue is Kinase

Cellular-based Assay

Technology Platforms

  • Cytotoxicity Assay
  • Apoptosis Assay
  • Cell Migration Assay
  • Cell Invasion Assay
  • Immunostaining Assay
  • Immunofluorescence Assay (96/384 wells)
  • Reporter Gene Assay (GFP/Luc)
  • Cellular Uptake Assay
  • Gene Knockdown Assay
  • RNAi Assay
  • MicroRNA Over-expression Assay
  • MicroRNA Knockdown Assay
  • Adenovirus/retrovirus/lentivirus

Cytotoxicity Assay Using celltiter glo

Cell-based Cathepsin Assay for irreversible inhibitors

Cell Cycle Analysis Using Flow Cytometry

Western Blot Analysis

Expression level of Phospho-Akt and Akt in mouse tumor

Migration Assay in HT1080 cells and MDA-MB-231 cells

Cytotoxicity Assay Using celltiter glo

Cell-based Cathepsin Assay for irreversible inhibitors

Pre-migration

Post-migration

Background

Positive control

Radioactive Assay

Technology Platforms (3H, 33P, 32P, 35S, 125I,14C)

  • Radioactive Receptor Binding Assay
  • Radioligand Binding Assay
  • Radioactive Uptake Assay
  • Cell Proliferation Assay (3H-TdR)
  • Radioimmunoassay (RIA)
  • Immunoradiometric Assay (IRMA)

Receptor binding assay-3H

3H-5HT uptake assay-3H

Radioactive iodine uptake assay-125I

Services

  • Chemistry Services
  • Biology Services
  • Bioanalytical Services
  • Drug Safety Evaluation
  • Formulation Development Services
  • Pharmacology Services
  • Pharmacokinetic Services
  • Integrated Projects

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About Us

  • Medicilon Overview
  • Executive Team
  • CEO Message
  • Company History
  • Corporate Culture
  • Honor

Services

  • Chemistry Services
  • Biology Services
  • Bioanalytical Services
  • Drug Safety Evaluation
  • Formulation Development Services
  • Pharmacology Services
  • Pharmacokinetic Services
  • Integrated Projects

Customer Center

  • Customer Reviews
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  • Intellectual Property Protection
  • New Models of Collaboration

Contact Us

Shanghai Medicilon Inc.

Address: 585 Chuanda Road, Shanghai  China
Email: Marketing@medicilon.com
Website: www.medicilon.com

Copyright 2017 Shanghai Medicilon inc. | All Rights Reserved
  • Languages
  • 中国日本대한민국
  • Home
  • About
    • Medicilon Overview
    • Executive Team
    • CEO Message
    • Corporate Culture
    • Company History
    • Honor
  • Services
    • Chemistry Services
      • Medicinal Chemistry Services
      • Synthetic Chemistry
      • Process Development Services
      • Analytical Chemistry
    • Drug Formulation
      • Pre Formulation Development
      • Analytical Development
      • Formulation Development Services
      • Generics Consistency Evaluation
      • Research on Quality Control
      • Clinical Trial Material
    • Biology Services
      • Discovery Biology Services
      • Recombinant Protein Services
      • Computational Biology & Molecular Modeling
      • Crystallization & Structural Determination
      • Kinase Services
      • SeMet Media Sales
    • Pharmacology Services
      • Cancer Xenograft Models
      • Orthotopic Models
      • Syngeneic Mouse Models
      • Transgenic Mouse Model
      • Humanized Mouse Model (hPBMCEngrafted)
      • PDX Model
      • Digestive System Models
      • Neurological Disorders Models
      • Metabolic Disease Models
      • Inflammation & Immunological Disease Models
      • Other Disease Models
    • Bioanalytical Services
      • Large Molecule Bioanalysis
      • Small Molecule Bioanalysis
    • Pharmacokinetic Services
      • In Vivo Assays
      • In Vitro Assays
    • Drug Safety Evaluation
    • Integrated Projects
      • Preclinical Research Services
      • IND Filing
      • Bio Drug Development
      • Chemical Drug Development
      • Chemical Compound Druggability Assessment
  • Customer
    • Customer Reviews
    • New Models of Collaboration
    • Intellectual Property Protection
    • Download Center
  • Careers
  • News
    • Our Events
    • Market Activity
    • Press Events
  • Contact
Medicilon Inc