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Baculovirus Expression System (Also Called Insect Cell Expression System)

2020-12-01
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Insect baculovirus expression system is one of the eukaryotic expression systems. It has the characteristics of high expression level of foreign genes, easy expression of heteromultimeric proteins, and high safety. The recombinantly expressed protein products have correct post-translational modification and biology Activities such as disulfide bond formation, phosphorylation, glycosylation, etc. It is suitable for the expression of macromolecular proteins. The insect expression system can express very large exogenous genes (up to 200kD). The expression system uses the expression vector constructed by the strong promoter of the polyhedrin protein in the baculovirus structural gene, which can make many The eukaryotic target gene is expressed effectively or even at a high level, and the highest expression level can reach 50% of the total insect cell protein.


Baculovirus expression vector system (Baculovirus expression vector system, BEVS) has been researched since its inception in 1983 (Smith & Summer, 1983; Maeda et al 1984), due to its high-efficiency expression ability, safety, and easy operation. As well as powerful and popular tools for producing various prokaryotic and eukaryotic proteins, a large number of foreign genes have been successfully expressed, covering almost all genes that can be developed and utilized by all species.

Medicilon’s Baculovirus Expression Vector System

Medicilon researchers establish a well-developed baculovirus-insect cell expression services platform. We provide the expression and purification services on preparation of recombinant baculovirus and recombinant protein. We have a good track record in producing kinase and recombinant protein complexes.

Service:

  1. Generation of recombinant Bacmid DNA

  2. Preparation of recombinant baculovirus

  3. Titration of baculovirus

  4. Protein expression verification and optimization

  5. Small scale expression and purification of recombinant protein in insect cell

  6. Scale up expression and purification of recombinant protein in insect cell

Related Articles:

Baculovirus Expression System

Baculovirus Expression Vector System

Introduction of Bac-to-Bac System

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