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CFDA basic requirements for non-clinical pharmacology/toxicology content Study on primary and secondary pharmacodynamics and mechanism of action ● First determine the registration classification. The newer the registration classification and the newer the target, the more research on drug efficacy is needed, and different animal models should be used to illustrate the clinical and mechanism feasibilityRead more

Medicines are the crystallization of important weapons for mankind to protect itself against the outside world; in its research process, many disciplines and systems engineering are refined. There are many pharmacological accidents caused by drug safety issues in history, and they also gave birth to many evaluation systems. Today we are going to talk aboutRead more

Immunofluorescence technique (Immunofluorescence technique, IF), also known as fluorescent antibody technique, is one of the earliest developed labeling immunological techniques. In 1941, Coons and others successfully used fluorescein to label antibodies for the first time and established immunofluorescence technology. After decades of development, the technology has become quite mature. Immunofluorescence technique The method of usingRead more

Recently, many fans have successively asked questions about “immunofluorescence” in the background, basically asking questions about immunofluorescence analysis. Here comes the problem. “Should immunofluorescence be analyzed?” Regarding this topic, I would like to discuss it carefully in a question-and-answer manner. In this way, people who read this article will not feel confused. Question 1: WhatRead more

Direct and indirect methods are not limited to immunofluorescence, they are also related to other technologies that rely on fluorescent dyes, enzymes or colored particles coupled to antibodies, such as flow cytometry, ELISA, and immunohistochemistry. But these two methods are different. As shown in the table below, both methods have their pros and cons: directRead more

What is immunofluorescence technology? Immunofluorescence technology is an immunoassay technique that combines the specificity of antigen-antibody reaction with the sensitivity and intuitiveness of fluorescent substance detection. What are the types of immunofluorescence technology? Fluorescent antibody staining technology: fluorescence microscopy technology, flow cytometry immunofluorescence technology (2) Fluorescence immunoassay: time-resolved immunoassay, fluorescence polarization immunoassay What are theRead more

Direct immunofluorescence technology combines immunological methods (antigen-antibody specific binding) with fluorescent labeling technology to study the distribution of specific protein antigens in cells. Because the fluorescence emitted by fluorescein can be detected under a fluorescence microscope, the antigen can be located on the cell. The method of using fluorescent antibody to trace or check theRead more

Introduction: Commonly used fluoresceins for flow cytometry detection include FITC, TRITC, Cy3, Cy5, PE and PI. Among the above various fluorescent dyes, PE has the strongest fluorescence and is suitable for weakly expressing antigens; FITC is the cheapest and is suitable for strong Express antigen, wide application range. 1 Flow cytometer (Flow cytometer) is aRead more

The standard technique for autoantibody diagnosis is indirect immunofluorescence, which is characterized by strong specificity and obvious signal intensity contrast between positive and negative samples. Microscopic observation can accurately determine the distribution of fluorescence in tissues or cells. The position of the autoantigen determines the typical fluorescence pattern of its corresponding antibody, and the stainingRead more

Autoantibody detection is an important method for diagnosing autoimmune disease (autoimmune disease, AID). Commonly used detection methods include indirect immunofluorescence (IIF), enzyme-linked immunoassay (ELISA), western blotting, etc., with the continuous innovation of biotechnology , Auto-antibody detection has been gradually applied to autoantibody detection by technologies such as magnetic bead particle chemiluminescence, immune microarray, and flowRead more