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Medicilon’s Stable Cell Line Service

2016-10-17
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Medicilon offers high quality, fast turnaround and cost-effective Stable Cell Line Service for protein expression, gene knockdown and genome editing that meet your specific research needs. Based on 12 years’ experience on cell line development, our cell culture scientists focus on optimizing all parameters specific to your cell lines to efficiently produce stable cell lines according to your requirements.

 

cell line services Stable cell line is the best solution, not only to produce recombinant protein and antibody, but also for application in drug screenings, gene functional studies etc. Depending on the type of gene expression and the gene construct, there are many different approaches for establishing stable cell lines.

We offer an array of services to simplify cell line development. From vector design to accession cell banking, our industry experienced scientists and project managers are able to provide support of biotechnology products destined for regulatory approval.  Medicilon offers the following assays on a fee-for-service basis:

• Sterility Testing
Toxicology Assay
• Mycoplasma Detection & Elimination
• Endotoxin Testing and Removal
• Stability Testing
• Cell Cycle
• Cell Apoptosis
• Cell Viability Studies ( MTT/ MST/ WST-8/ WST-1 and other assays)
ELISA
Western Blot 

Cell Line Stability Evaluation

Cell line stability is a critical factor for process scale up.  For large-scale commercial manufacturing, cell lines routinely grow for over 25 generations to achieve high-density culture in the 10,000-20,000 liter production bioreactor. Studies to determine cell line stability are performed up to 50 generations. Best performing cell line will be propagated in small suspension culture and scaled to the 5-L bioreactor at interim time points to evaluate cell growth and product yield and determine long-term cell line stability.

This service can be done as follows dependent upon each quote:

• Make your Lentiviral inducible shRNA vector for your targets
• Delivery purified, Ready-To-Use lentivirus for your target of interest at high titer around 10e7 IFU (Infection Function Units)/ml
• Generate stable cells in the almost any cell line your desired
• Protein expression in suspension mammalian cells using lentviral transduction
Purification of mammalian cell expressed proteins by affinity column (His-tag)

Medicilon has extensive experience in the field of cell culture and cell line generation. Our scientists have developed several perfect stable cell lines and also produced a large number of antibodies and proteins through stable cell lines. Our specific biosimilar cell lines and hybridoma cell lines are available in-house, and we are also pleased to provide custom strains to meet our clients’ individual requirement.

Contact Us 

Email : marketing@medicilon.com

Tel : +86 021 58591500

 

Tips : Above is part of cell line service,stable cell line development,stable cell line generation. You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

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