(1) Protein binding: plasma, brain tissue, microsomal protein, FBS; Erythrocyte /plasma partition ratio
(2) Optimization permeability and transshipment: Caco - 2. MDCK - MDR1 / BCRP, OATs/OCTs/OATPs
(3) In vitro metabolic DDI: P450 inhibited TDI, P450 induced /PXR, metabolic enzyme phenotype.
(4) Metabolic stability: microsomes, S9, hepatocytes, plasma, whole blood
(5) Metabolite Screening and Identification: in vitro, in vivo, and GSH Trapping
(6) In vivo PK: rat, mouse, dog, monkey, pig, rabbit box/single administration
--- IV/IJVC/SC/TD/IM/IP/PO/SL/IN/IVT
--- Continuous cross/single point blood sampling
--- BBB(homogenate /CSF), Tissue distribution excretion (BDC), in vivo DDI (ABT)