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HPLC Method Development Service

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Chemical Analytical Center of Medicilon provides general analysis and purification services for the clients. Our experts have rich experience and proficient skills to solve problems, which are highly appreciated by clients.

General Analytical Chemistry Services

– LC-MS Analysis
– HPLC Analysis (including ELSD)
– Chiral HPLC Analysis

– General Testing (ROI、LOD、Cl-、SO42-、mp、HM、Specific Rotation、Water Content, et al)

For more detail,please click link Analytical Chemistry.

High Performance Liquid Chromatography (HPLC) is an analysis technique used for separation and quantification of small molecules and polymers.  HPLC is the method of choice for testing complex mixtures containing non-volatile and semi-volatile organic compounds such as polymer additives, active pharmaceutical ingredients (APIs) and impurities.

HPLC analysis service hplc method development

What is HPLC (High Performance Liquid Chromatography)?

• HPLC is a separation technique that involves:

– the injection of a small volume of liquid sample
– into a tube packed with tiny particles (3 to 5 micron (µm) in diameter called the stationary phase)
– where individual components of the sample are moved down the packed tube (column) with a liquid (mobile phase) forced through the column by high pressure delivered by a pump.

• These components are separated from one another by the column packing that involves various chemical and/or physical interactions between their molecules and the packing particles.

• These separated components are detected at the exit of this tube (column) by a flow-through device (detector) that measures their amount. An output from this detector is called a “liquid chromatogram”.

The Method of High Performance Liquid Chromatography (HPLC)

•  An analytical separation technique that involves the high-pressure flow of a liquid through a column that contains the stationary phase.

Mobile phase: Liquid
Stationary phase: Can be a solid (LSC) or a liquid (LLC)

•  A mixture of compounds injected at one end of the column separates as the compounds pass through.

•  Separated compounds are detected electronically as they elute at the other end of the column.

Application of HPLC (High Performance Liquid Chromatography

General Application

• Separation of organic, inorganic, biological compounds, polymers, and thermally labile compounds
• Qualitative and quantitative methods

Common Specific Applications 

• Quantitative/qualitative analyses of amino acids, nucleic acids, proteins in physiological samples
• Measuring levels of active drugs, synthetic byproducts, degradation products in pharmaceuticals
• Measuring levels of hazardous compounds such as pesticides and insecticides
• Monitoring environmental samples
• Purifying compounds from mixtures


• Qualitative analysis may be limited unless HPLC is interfaced with mass spectrometry
• Resolution is limited with very complex samples

Complementary or Related Techniques

• Gas chromatography provides analyses volatile analytes with superior resolution
• Supercritical fluid chromatography provides analyses of volatile, non-volatile and thermally labile compounds
• Capillary electrophoresis provides superior analyses in many biological/pharmaceutical applications

• Ion Chromatography provides analyses of ionic compounds, as does capillary electrophoresis

Contact Us 

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Tel : +86 021 58591500

Tips : Above is part of HPLC analysis service, HPLC method development, HPLC testing labs and HPLC price . You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

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