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Mammalian Protein Expression System

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Mammalian protein expression systems are the best choice for the production of eukaryotic proteins, especially when correct folding and post-translational modification is required. They produce eukaryotic recombinant proteins in the most natural state, with native tertiary structure, physiochemical characteristics and bioactivities. They have been successfully applied in the biopharmaceutical production of cytokines, monoclonal antibodies, growth factors and so on.

Mammalian expression

Medicilon offers a variety of protein expression systems from small scale to large scale with a full-range of purification options. Our services include: gene synthesis, generating expression construct, establishing stable cell line, generating recombinant virus, small-scale expression and purification, large scale expression and purification including bacteria, baculovirus/insect, yeast and mammalian cells. Our Mammalian Expression System offers flexibility you need to express and produce the recombinant protein of your choice.

Our Mammalian Expression Service:

CHO, Vero, BHK, 293 Cells etc
Adherence and Suspension Culture
Transient Expression Screen
Stable Cell Line Generation
Rabbit IgG Expressed in Transient Transfected 293 Cells

Featuring protein folding and post-translational modification, mammalian protein expression systems can make proteins that are closer to the natural proteins, thus obtaining the same biological activity and characteristics. Therefore, the mammalian cell expression system has been widely applied in the R&D and production of recombinant protein drugs, especially therapeutic recombinant monoclonal antibody drugs.

Based on a serum-free culture system, our mammalian protein expression system offers various eukaryotic protein services, including gene expression optimization, transient expression, stable expression, and antibody production. Our researchers have established a mature mammalian protein expression service platform to provide expression and purification of mammalian proteins.

Mammalian Service Process

Step 1. Bioinformatic Analysis, Domain Selection and Vector Design
Step 2. Construction of Expression Vectors
Step 3. Cell Transfection, Selection and Cloning of Target-Expressing Cells
Step 4. Protein Production and Purification
Step 5. Scale-Up Production and Quality Control Test
Step 6. Characterization of Target Protein (Including Functional Studies)

Mammalian Expression Features

Protein Production with Accurate Post-Translational Modification
Bioactive Protein Production
Transient and Stable Expression
Large-Scale Protein Production
Each protein has its own biochemical features and differences in the expression process.  Medicilon’s team works with our clients to develop the best strategy and use the most cost-effective processes. Our system is designed to best accommodate the needs of our clients and has been used to successfully produce many difficult proteins.

Contact Us 

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Tel : +86 021 58591500

Tips : Above is part of mammalian protein expression system, protein expression in mammalian cells . You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

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