Commonly Used Mouse and Nude Mouse Tumor Model

1、Research on the Level of Animal Tumor Transplantation Model

In the preliminary screening of antitumor drug pharmacodynamics or the evaluation of preliminary efficacy, the mouse tumor transplantation model was used in the in vivo test. In animal tumor transplantation models, Kunming mice or purebred mice are often used to inoculate mouse tumors, and the inhibitory effect of the test substance on tumor growth is observed. The mouse tumor xenograft model has the advantages of short experimental period, good repeatability, small space required, relatively low cost, quantitative analysis and different treatment plans or conditions according to different tumors, so it has been widely used.

The list of commonly used mouse tumor xenograft models is as follows:

2、Research on the Level of Human Cancer Xenograft Model

In the evaluation of the effectiveness and reliability of the pharmacodynamics of antitumor drugs, human cancer xenograft models are used in in vivo experiments. Human cancer xenograft models are usually transplanted with human cancer cell lines in athymic mice or combined immunodeficient mice. Inhibitory effect of test substance on tumor growth. Nude mice are homozygous (nu/nu), hairless, and athymic. Due to its congenital defect in the thymus, T cells are deficient and readily accepted for xenotransplantation. However, due to its low immune function, it is susceptible to microbial infection, and it is not easy to survive under conventional conditions, with an average lifespan of only about 30 days. Under sterile conditions, the lifespan of nude mice can be close to that of normal mice. The best way to raise nude mice is to keep them under germ free (germ-free) conditions, but this condition is generally unattainable; the second is to keep them under specific pathogens free (SPF) conditions. In 1969, Danish scholar Ryaard successfully transplanted human colon cancer into nude mice for the first time, which was the first successful human tumor xenotransplantation. Nude mice have now been recognized as ideal animals and important tools for human cancer models, and are known as "living test tubes". The xenotransplantation of human cancer generally does not require any additional treatment to the animal, the tumor can grow, and can be passaged in vivo for a long time to maintain its original biological characteristics. Due to the strong correlation between human cancer xenograft models and clinical efficacy, the effectiveness and reliability of antitumor drugs are usually evaluated by the results of human cancer xenograft models. The list of commonly used nude mouse tumor xenograft models is as follows: