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Top 10 Global Clinical Research Organizations in 2021| Top 10 Global CDMO Enterprises| Preclinical Drug Development Process
Aug 09,2021
What is IND, NDA, ANDA?
The FDA new drug review process includes two processes: ind filing for new drug clinical trial application and new drug application NDA application. After the applicant completes the preclinical study of the new drug, he can submit an IND filing to the FDA. If the FDA does not object within 30 days of receipt ApplicantsRead more
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What is IND, NDA, ANDA?
Aug 06,2021
General Principles and Method Selection of Protein Purification
With the development of molecular biology, more and more scientific researchers have mastered various experimental techniques of molecular biology, and developed a complete set of kits to make gene cloning and expression easier and easier. However, the upstream work of molecular biology is often not the ultimate goal. The key to molecular cloning and expression
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General Principles and Method Selection of Protein Purification
Aug 05,2021
Cell and Gene Therapy: a New Direction for Innovative Therapies
Cell therapy Cell therapy is the introduction of new cells into a tissue to stimulate the body’s ability to heal itself and repair or rebuild a strong immune system in order to treat disease. Cell therapy is mainly divided into stem cell therapy and immune cell therapy. Stem cells therapy Stem cells have the abilityRead more
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Aug 04,2021
What is Bioanalysis
Biological analysis refers to the quantitative analysis of target substances in biological matrix by chromatography, chromatography-mass spectrometry, ligand binding and other techniques. Bioanalysis includes the analysis of drugs, metabolites, proteins, peptides and other large and small molecules in the matrix. In the pharmaceutical industry, bioanalysis has been widely used in the process of drug discoveryRead more
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What is Bioanalysis
Aug 04,2021
The experimental principle and operation steps of ELISA
1. Experimental principle ELISA is a highly sensitive test technique based on immunological reaction, which combines the specific reaction of antigen and antibody with the efficient catalysis of enzyme to substrate. Since the reaction of antigen and antibody is carried out in a solid phase carrier — polystyrene microtitration plate, after each addition of aRead more
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The experimental principle and operation steps of ELISA
Aug 04,2021
ELISA Experiment Guide: Indirect ELISA
Indirect ELISA Indirect ELISA is illustrated in the figure below and Figure 2. Phases I and II are similar to the direct system.Phase III involves adding unlabeled detection antibodies that are diluted in the buffer to prevent the proteins in the antiserum from nonspecifically attaching to the solid phase (the blocking buffer). It is thenRead more
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Aug 03,2021
ELISA Indirect Method
As we all know, enzyme-linked immunosorbent assay (ELISA) is a technique that uses antigen-antibody interaction to quantify proteins, hormones, antibodies or viral antigens. Up to now, more than 200 research papers related to ELISA and antibody detection have been published in journals around the world every year. Among them, there are more than 70 papersRead more
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ELISA Indirect Method
Aug 03,2021
Indirect ELISA procedure
Indirect ELISA procedure-Experimental materials 1. 96-well microplate 2. TMB Experimental reagents 1. Encapsulation solution (pH9.6) : Na2CO3 0.17g, NaHCO3 0.28g, add distilled water to 100ml.4 ℃. 2. Washing solution (PBST): KH2PO4 0.2g, KCl 0.2g, NaCl 8.0g, Na2HPO4·12H2O 2.9g, Tween-20 0.5mL, add deionized water to 1000mL, adjust pH to 7.2-7.4. 3. Substrate reaction liquid: (1) SubstrateRead more
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Indirect ELISA procedure
Aug 02,2021
Drug cardiotoxicity research technique
Cardiotoxicity caused by some drugs during use is one of the life-threatening toxic side effects of patients. For example, adverse cardiac events caused by antiarrhythmic drugs have been reported from time to time. Although the incidence of such events is low, the risk is high, and the main manifestations are To prolong the QT intervalRead more
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Drug cardiotoxicity research technique
Aug 02,2021
Drug Safety Evaluation
Medicilon has a professional team and practical experience in drug safety evaluation, and can provide high-quality data and fast turnaround to support various drug safety evaluation studies. Toxicology studies can be performed in accordance with non-GLP or GLP standards. Our research platform has been rated as the Shanghai R&D public service platform. Medicilon drug safetyRead more
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Drug Safety Evaluation