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Top 10 Global Clinical Research Organizations in 2021| ADC, precision and deep cultivation for targeted drug delivery| What are the three major in vitro pharmacokinetic research methods?
Apr 06,2021
Generic drug consistency evaluation is not that simple
Since 2015, my country has initiated the process of in-depth reform of the drug review and approval system. "Improving the quality of generic drugs and accelerating the quality consistency evaluation of generic drugs” has become one of the five major goals of the reform. Before the end of 2018, the completion of the quality consistencyRead more
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Apr 06,2021
Generic drugs that pass the consistency evaluation are trustworthy
China's consistency evaluation standards are strict. Q1 The generic drug and the original drug have the same common name and same chemical composition. Why is there a difference in quality? What impact will this difference have on the clinic? My country is a big country of generic drugs. Previously, my country used imitation standards whenRead more
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Apr 02,2021
Details of success or failure in ELISA experiment operation
Enzyme-linked immunosorbent assay (ELISA) is widely used in the determination of various antigens and antibodies in clinical testing because of its high sensitivity and strong specificity. Although the ELISA operation is simple, there are also big articles in the small experiment. Each link of the ELISA operation has a great influence on the detection effectRead more
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Details of success or failure in ELISA experiment operation
Apr 02,2021
Influencing factors of wrong results of ELISA
Influencing factors of wrong results of ELISA ELISA, or enzyme-linked adsorption test, is a commonly used solid-phase enzyme immunoassay method. Engvall and Perlmann first applied this method to quantify IgG in 1971, and named it “enzyme linked immunosorbent assay (ELISA)“. ELISA The basic principle of ELISA is: ① Make the antigen (or antibody) bind toRead more
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Apr 02,2021
Common problems in ELISA experiment operation
ELISA, or enzyme-linked immunosorbent test, is an experimental method commonly used in hospital laboratory and scientific research laboratories. It has high sensitivity and specificity and is widely used in clinical practice. Mainly used for qualitative or quantitative determination of antibody or antigen concentration. The experimental operation includes several main steps such as sample loading, plateRead more
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Apr 02,2021
ELISA technology’s technical details
ELISA: The full name is enzyme-linked immunosorbent test. Its principle is: during the measurement, the test specimen reacts with the antigen or antibody on the surface of the solid-phase carrier, and the antigen-antibody complex formed on the solid-phase carrier is washed into the solution. Separate the other substances, add enzyme-labeled antigen or antibody, and bindRead more
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Apr 01,2021
Key points of ELISA experiment: washing is the key
One of the important points of optimizing ELISA is washing. The washing step reduces the background signal caused by unbound antibody, thereby increasing the signal-to-noise ratio of the analysis. The washing between each step ensures that only specific binding events are retained, generating a signal in the last step. Insufficient washing can lead to differencesRead more
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Apr 01,2021
The operation points of ELISA technology
High-quality reagents, good instruments and correct operation are necessary conditions to ensure the accuracy and reliability of ELISA test results. The operation of ELISA differs due to the formation of solid-phase carriers, and domestic medical tests generally use plate-type dots. This article will describe the main points of attention in each operation step of plateRead more
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The operation points of ELISA technology
Apr 01,2021
ELISA is always unsuccessful, what is wrong?
ELISA has always been widely used clinically because of its high sensitivity and good specificity. As a classic immunoassay method, although it seems ordinary, it is not easy to do the experiment well. The reading of the ELISA plate will always be unsatisfactory. It can be seen that each link in the actual operation ofRead more
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Apr 01,2021
9 tips for correct ELISA operation
Clinical ELISA assays are usually manually operated with microwell plates as the solid phase. The assay operation is very simple, generally involving specimen collection and preservation, reagent preparation, sample addition, incubation, plate washing, color development, In terms of colorimetry, result judgment, and result reporting and interpretation, improper steps in any of these steps will affectRead more
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