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Top 10 Global Clinical Research Organizations in 2021| ADC, precision and deep cultivation for targeted drug delivery| What are the three major in vitro pharmacokinetic research methods?
Oct 28,2021
Application of Yeast Expression System
Background Yeast expression system is the most economical and efficient eukaryotic protein expression system, which can successfully achieve intracellular expression or secretory expression, and its amplification medium is relatively cheap, and the culture conditions are not demanding, so it is suitable for industrial amplification. Like mammalian cell expression systems, yeast protein expression systems can performRead more
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Application of Yeast Expression System
Oct 28,2021
Yeast Expression System and Method
The Production of Yeast Expression System The development of genetic engineering technology has shown broad prospects for the synthesis and production of foreign proteins by microorganisms. For a long time, people have used E. coli as a host to express a variety of proteins. This is because E. coli has several advantages, such as clearRead more
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Yeast Expression System and Method
Oct 27,2021
Immunofluorescence Technique
Immunofluorescence technique (immunofluorescence technique) is also known as fluorescent antibody technique. Founded in the early 1940s, in 1942, Coons et al. reported several times that antibodies were labeled with fluorescein isocyanate. It is a technology established on the basis of immunology, biochemistry and microscopy. Immunofluorescence technology includes fluorescent antibody technology and fluorescent antigen technology, becauseRead more
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Immunofluorescence Technique
Oct 27,2021
Direct and Indirect Fluorescent Antibody Technology
Direct Fluorescent Antibody Technology The direct method is the simplest and most basic method of fluorescent antibody technology. It uses the labeled antibody to directly bind to the corresponding antigen (antigen specimen to be tested) to identify the unknown antigen. Advantages: ① Since there are only two factors involved in the reaction, the result judgmentRead more
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Direct and Indirect Fluorescent Antibody Technology
Oct 26,2021
Common Protein Expression System
Introduction In vitro recombinant protein production is a protein obtained by applying recombinant DNA or recombinant RNA technology. Catalog The use of in vitro protein expression technology to extensively produce various types of recombinant proteins, which are used in the fields of medicine, food, daily chemical and other fields. The following briefly introduces the mainRead more
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Oct 26,2021
Application of Baculovirus-insect Cell Expression System
Introduction to Bac-to-Bac System The baculovirus-insect cell system is a binary system consisting of two parts. The first part is the baculovirus expression vector, which is an insect virus whose function is obviously to introduce the foreign gene encoding the target protein into the host cell. Another function of baculovirus expression vectors is to provideRead more
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Oct 26,2021
Comparison of E. coli and Insect Baculovirus Expression Systems
Principle of Escherichia coli Expression System The Escherichia coli expression system is an expression system that efficiently expresses genes encoding protective antigens of pathogenic microorganisms in Escherichia coli through DNA recombination technology. It is currently the most commonly used system for recombinant protein expression. The E. coli expression system has been studied earlier and hasRead more
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Oct 25,2021
Baculovirus Expression Vector System and its Application
The most researched insect baculovirus is Autographa californica multiple nuclear polyhedrosisvirus (AcMNPV), its host is Spodoptera frugiperda (Sf); Bombyx mori nuclear polyhedrosis virus (Bombyx) mori nuclear polyhedrosis virus (BmNPV), whose host is the silkworm (Bombyx mori), these two baculovirus vectors are often used to express foreign proteins [2,3]. The gene expression of baculovirus is dividedRead more
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Oct 25,2021
What is Baculovirus Expression System and its Advantages
What is Baculovirus Expression System Baculovirus belongs to the Baculoviridae family (Baculoviridae), which is divided into nuclear polyhedrosis virus and granulovirus. The virus particles are rod-shaped and are a type of DNA virus that specifically infects arthropods and has an envelope. Genomic DNA is compressed and packed in a rod-shaped capsid in a super-coiled form,Read more
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What is Baculovirus Expression System and its Advantages
Oct 22,2021
What is an API Drugs?
What is an API Drugs? API drugs refer to the raw materials used in the production of various preparations. They are the effective ingredients in the preparations. Various powders, crystals, extracts, etc., prepared by chemical synthesis, plant extraction or biotechnology, are used for medicinal purposes. Substances taken directly. APIs, also known as active ingredients ofRead more
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