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Study on the Pharmacological Effects of Dendrobium Nobile and its Pharmacokinetics in Rats

2021-09-15
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Dendrobium nobile has a long history of medicinal use. It is a common traditional Chinese medicine. The chemical components are divided into alkaloids, glycosides, phenanthrene and bibenzyls according to the structure type. Its pharmacological effects are mainly manifested in anti-tumor, hypoglycemic and anti- Nervous system protection and other aspects. The pharmacokinetic study of dendrobine in normal rats found that dendrobine has a good separation from endogenous impurities, and the retention time of dendrobine is about 4.0 min. The pharmacological and pharmacokinetic studies of Dendrobium nobile can provide scientific basis for the drug development and clinical application of this medicinal material.

Pharmacology is a science that studies the law of interaction between drugs and the body and the mechanism of drug action. It mainly includes two aspects: pharmacodynamics and pharmacokinetics. Pharmacokinetics is a subject that quantitatively studies the absorption, distribution, excretion and metabolism of drugs in organisms. Medicilon provides pharmacokinetic research services. Its pharmacokinetic laboratory has passed CFDA’s GLP certification. The research follows the guiding principles of ICH, CFDA and FDA, and can design and carry out in vivo and in vitro pharmacokinetic tests according to customer needs, and provide customers with a complete set of pharmacokinetic evaluation and optimization services.

Study on the Pharmacological Effects of Dendrobium Nobile and its Pharmacokinetics in Rats

In recent years, many scholars have studied the pharmacological activity and clinical application of the components isolated from various Dendrobium plants. Studies have shown that Dendrobium plants have anti-tumor, anti-mutation, enhancement of body immunity, anti-aging and lowering blood sugar. And so on.

The pharmacological effects of Dendrobium nobile

(1) Anti-tumor effect

The phenanthrene and bibenzyl substances extracted from Dendrobium nobile, not only have anti-lung cancer, ovarian adenocarcinoma and promyelocytic leukemia effects, but also promote the growth of T cells and the production of inhibitory factors by lymphocytes to enhance immunity. . The ethyl acetate extract of Dendrobium nobile has significant cytotoxic effects on human lung cancer cells, human ovarian adenocarcinoma cells, and human promyelocytic leukemia. In particular, it has a great inhibitory effect on lung cancer cells, and the inhibition rate is as high as 74.7%-97.2%. Two compounds, Lusianthridin and denbinobin, isolated and identified from the crude extract of Dendrobium have the same pharmacological effects as the crude extract, and the former also has an inhibitory effect on transplanted sarcoma S-180.

(2) Anti-aging and improve immunity

Dendrobium nobile can significantly increase the level of superoxide dismutase (SOD), reduce lipid peroxide (LPO), regulate the level of brain monoamine neuromediators, inhibit similar monoamine oxidase (MAO), and delay aging.

(3) Promoting blood circulation and removing blood stasis, improving cardiovascular and cerebrovascular functions

Dendrobium nobile contains esters, which can promote blood circulation, remove blood stasis, dilate blood vessels and resist platelet coagulation, and treat thrombo-occlusive vasculitis, cerebral thrombosis, and arteriosclerotic occlusion. Mailuoning injection extracted from Dendrobium, Honeysuckle and other medicinal materials has the functions of clearing away heat and nourishing yin, promoting blood circulation and removing blood stasis.

(4) Treat digestive system diseases

Dendrobium nobile has an excitatory effect on the intestinal tube and can increase the contraction range. Pharmacological studies have shown that oral dendrobium decoction can promote gastric juice secretion and help digestion. Dendrobium and fexetine are similar in efficacy but weaker. Dendrobium extract can stimulate the contraction of the smooth muscle of the small intestine and promote gastrointestinal peristalsis. It can receive unique and satisfactory results for chronic atrophic gastritis.

Study on the pharmacokinetics of Dendrobium nobile in SD rats

The content of dendrobine in rat plasma was detected by UPLC-MS method, and the pharmacokinetic characteristics of Dendrobium nobile powder in rats after intragastric administration were investigated. To establish a method for the analysis of dendrobine in plasma samples, rats were given 0.8 g/kg of Dendrobium nobile powder by gavage at 0, 2, 6, 10, 15, 20, 30, 40, 60, 90, 120, Rat plasma was collected at 180, 240, 480, 720, 1 440 min. After sample processing, the thermo Q Exactive quadrupole-electrostatic field orbitrap high-resolution mass spectrometry system was used for analysis. Hypersil Gold C18 column (150 mm×2.1 mm, 1.9 μm) was eluted with a gradient of 0.1% formic acid aqueous solution-acetonitrile, and the flow rate 0.3m L/min, ESI source positive ion detection of dendrobium content, pseudoephedrine hydrochloride as internal standard.

The results found that the endogenous substances in rat plasma samples did not interfere with the determination of dendrobine content, the methodological investigation met the requirements, and the established analytical method could meet the preclinical pharmacokinetic research. The pharmacokinetic results showed that the average peak time of plasma dendrobine concentration was 11.71 min, the average maximum plasma concentration was 212.17μg/L, and the average half-life was 351.95 min.

With dendrobine as the detection index, the pharmacokinetic characteristics of Dendrobium nobile powder in rats after intragastric administration were studied, which provided a reference for its further research and development. Dendrobium, as a commonly used precious Chinese medicine, has a history of more than 2,000 years of application. It has a relatively systematic preclinical research and clinical research basis. Its pharmacological activity is wide, and the curative effect is relatively stable and reliable. It provides important for the modern application of this Chinese medicinal material Basis.

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