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Stable Cell Line Generation Service

2017-05-25
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Shanghai Medicilon Inc. can generate your over-expressing or knockdown stable cell line with the best quality and best price.  We provide stable cell line generation service for research, biotechnology and pharmaceutical companies. Contact us via email : marketing@medicilon.com

stable cell line generation

Custom Stable Cell Line Generation Service Proceeds:

 

Phase I. Construct Generation (Optional)

Our professional scientists will generate constructs for your project. We will clone your target gene into lentiviral transfer vector. Lentivirus will be produced, concentrated and titrated after the constructs are confirmed by sequencing. Phase I takes about 3-4 weeks. However, we can also generate non-lentiviral vector constructs for your project if you need.

 

Phase II. Stable Cell Clones Selection

Perform transduction on target cells, select stable transduced cell clones. Phase II takes about 1-3 weeks depending on cell growth and antibiotics for selection.

Phase III. Gene Expression Evaluation
To evaluate the gene expression in stable transduced cell clones, our scientists will perform one or several assays including Western Blot, ELISA, real-time PCR or reporter assays, etc. Phase III usually takes about 2 weeks.

The services provided by AMSBIO for generation of cell lines include:

  • Integrating the desired gene or shRNA into the lentiviral vector

  • Generating the lentivirus particle with the desired gene

  • Transduce or infect the gene into the cell line needed

  • Selection of only high expression cells

  • Validate the integration of the gene by genomic PCR and validate the high-expression clone by Western Blot (if needed)

  • Preparation of two cryogenic preserved vials of stable cells along with documentation

Stable cell line is the best solution, not only to produce recombinant protein and antibody, but also for application in drug screenings and gene functional studies, etc. Depending on the type of gene expression and the gene construct, there are many different approaches for establishing stable cell lines.

Contact Us 

Email : marketing@medicilon.com.cn
Tel : +86 021 58591500
Tips:  Above is part of PCR Primer Design.  You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

Contact Us 

Email : marketing@medicilon.com
Tel : +86 021 58591500
Tips:  Above is part of stable cell line generation service and generation of stable cell lines.  You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

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