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Three Chemicals to Construct a Ulcerative Colitis Model

2020-12-02
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Ulcerative colitis is a lifelong immune disease of the intestine, also known as ulcerative colitis, and Crohn’s disease is collectively called inflammatory bowel disease. The ideal ulcerative colitis model is of great significance for elucidating the etiology and pathogenesis of the disease and exploring the therapeutic effects of drugs. Ulcerative colitis is an inflammatory process confined to the colonic mucosa and submucosa. The lesions are mostly located in the sigmoid colon and rectum. Dextran sodium sulfate (DSS) can cause animal ulcerative colitis models. BALB/c mice are sensitive to DSS-induced ulcerative colitis, and the lesion site is similar to humans (sigmoid colon and rectum), so it can be an ideal animal for experimental research.

DSS is a sulfated polysaccharide synthesized from sucrose, which has the same anti-hemostatic and anticoagulant effects as heparin. The induced animal model is widely used in experimental research. The intestinal pathology of the mouse ulcerative colitis model induced by DSS is most similar to the pathological changes of human ulcerative colitis, so it is widely used. In addition, the exogenous chemical irritants trinitrobenzene sulfonic acid, oxazolone, and DSS can all be used to construct BALB/c mouse ulcerative colitis model to study the etiology and pathogenesis of ulcerative colitis And exploring the efficacy of drugs.

Ulcerative colitis is a chronic non-specific intestinal inflammatory disease that mainly affects the rectum and colon. As the disease progresses, it can become colorectal cancer, which is called colitis-related colorectal cancer. To study ulcerative colitis, the first step is to establish an animal model of ulcerative colitis. In the field of inflammation and immune system diseases, the drug efficacy evaluation system of Medicilon includes models and evaluation methods for immune arthritis, psoriasis, atopic dermatitis, ulcerative colitis, and various acute and chronic inflammatory diseases.

It has been reported in the literature that 2,4,6-trinitrobenzene sulfonic acid (TNBS), oxazolone (oxazolone) and dextran sulfate sodium (DSS) can all induce acute and chronic colitis models. It is generally believed that the experimental enteritis models induced by trinitrobenzene sulfonic acid and sodium dextran sulfate correspond to Crohn’s disease (CD) and ulcerative colitis (UC), respectively. Some researchers have improved 2, 4, 6 Trinitrobenzene sulfonic acid (TNBS) silica gel tube enema induces the preparation of mouse ulcerative colitis model, which improves the success rate of modeling and the stability of the model, and explores the appropriate dosage and time for modeling. By making relevant technical improvements to the mouse UC model, the enema is more convenient, the efficiency of model building is improved, and the stability of the model is significantly increased.

Oxazolone is a hapten agent that causes severe colitis after intrarectal administration in mice. Some researchers also explored the establishment of oxazolone-induced ulcerative colitis (UC) model in mice, and evaluated its value in UC research [1]. The researchers randomly divided 30 BALB/C mice into model groups, The control group and the treatment group have 10 animals in each group. After oxazolone was given for modeling, the treatment group was given sulfasalazine by gavage, and the disease activity index (DAI) of the three groups of mice was observed. All the mice were sacrificed one week later, and the diseased colon was taken to observe the histological changes, and the weight of the spleen tissue was measured. The serum interleukin IL-10 and IL-2 levels of the three groups of mice were determined by enzyme-linked immunosorbent assay (ELISA). The results of the study found that oxazolone can induce an experimental mouse model similar to human UC. It can be used as an ideal model for studying the etiology, pathogenesis and drug treatment of UC.

There are reports in the literature that chemically induced colitis models are relatively easy and fast to establish and can be used with wild-type mice. Therefore, these models have been widely used to screen potential therapeutic substances.

[1] Experimental study on oxazolone induced ulcerative colitis in BALB/C mice[J]

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