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3D Cultured Spheroid Models

3D Cultured Spheroid Models

For cancer drug efficacy evaluation, Medicilon has developed 3D cel culture models with KRAS cell lines and associated diseases. Utilizing Medicilon's Ready-to-Use KRAS 3D Spheroid Models can enhance your preclinical research.
Paradigm Shifts from Animal Testing to Alternative Preclinical Drug Screening
3D Cultures: A Better Tool for In Vitro Cell-Based Assays
  • What is 3D Cell Culture:
    3D cell culture is a commonly used method for culturing cell spheroids, representing matrix-free 3D cell culture.
    What is 3D Cell Culture.webp
    Type of culture2D3D
    In vivo-likeNo mimicry of natural tissue or tumor structureResembles in vivo tissues and organs
    ProliferationFaster growth in monolayerSimilar to in vivo situation
    PolarityPartial polarizationAccurate cell polarization depiction
    Cell morphologySheet-like, flat cellsForm aggregate/spheroid structures
    Cell-cell interactionLimited interactionsProper cell-cell and cell-extracellular matrix interactions
    Gene/protein expressionAltered gene expressionRelevant gene/protein expression in 3D models
    Drug responsesLack of correlation with human tumorsSimilar drug resistance patterns as in patients
    Access to compoundsUnlimited access to nutrients and moleculesVariable access similar to in vivo conditions
    Testing durationShort-term acute toxicity assessmentLong-term low-dose repetitive exposure
Utilization of 3D Cultured Scaffold-Free Spheroids in Preclinical Studies
  • The Applications of 3D Cultured Scaffold-Free Spheroids in Preclinical Studies
    Drug screening and efficacy testingBetter predictive value for drug response compared to 2D culture systems.Evaluate toxicity, drug penetration, and therapeutic effectiveness.Disease modeling and understanding:Mimic the pathological features of diseases.Study disease progression and mechanisms.Tissue engineering and regenerative medicine:Develop bioengineered tissues for transplantation and organ-on-a-chip systems.Testing biocompatibility and functionality of engineered constructs.
    The Advantages of 3D Cultured Scaffold-Free Spheroids in Preclinical Studies
    Improved physiological relevance.Reduction in animal experimentation.Cost-effective and high-throughput screening.
Medicilon's Breakthrough: 3D Cultured Spheroids for Advanced Drug Safety Evaluation
  • Drug toxicity screening and efficacy testing:
Harnessing the Power of 3D Cultured KRAS Spheroids for Cancer Drug Efficacy Evaluation
  • Harnessing the Power of 3D Cultured KRAS Spheroids for Cancer Drug Efficacy Evaluation.webp
    KRAS mutations are frequently observed in various cancers and have been linked to cancer development, poor prognosis, and increased resistance.
  • Empowering Preclinical Studies: Ready-to-Use KRAS 3D Spheroid Models Developed in Medicilon
    Overview of 3D Spheroid Models Developed with KRAS Cell Lines and Associated Diseases
    Cell lineFull NameOrganKRAS mutant
    SW837Human colorectal cancer cellsColonG12C
    HCT15Human colorectal cancer cellsColonG13D
    LoVoHuman colorectal cancer cellsColonG13D
    HCT116Human colorectal cancer cellsColonG13D
    NCI-H23Human non-small cell lung cancer cellsLungG12C
    Calu-1Human lung cancer cellsLungG12C
    NCI-H358Human non-small cell lung cancer cellsLungG12C
    HPAF-IIHuman pancreatic cancer cellsPancreasG12D
    AsPC-1Human pancreatic adenocarcinomaPancreasG12D
  • Evaluating the Impact of KRAS Mutations on In Vitro Colon Cell Models Exposed to 5-Fluorouracil
    Evaluating the Impact of KRAS Mutations on In Vitro Colon Cell Models Exposed to 5-Fluorouracil.webp
    The study utilized four colon KRAS mutation cell lines to evaluate the comparative 5FU exposure results. The IC50 values of both 2D and 3D cultured cells were measured by ATP assay at various time points (24, 48, 96, and 168 hours), providing valuable insights into the efficacy of 5FU treatment in different cell culture models.
  • Investigation of Drug Effects on In Vitro NSCLC Cell Models with G12C KRAS Mutations
    Investigation of Drug Effects on In Vitro NSCLC Cell Models with G12C KRAS Mutations.webp
    The study investigated the effects of doxorubicin (DOX), gemcitabine, and AMG510 on multiple in vitro non-small cell lung cancer (NSCLC) cell models carrying the G12C mutation within the KRAS gene.IC50 values were determined based on the ATP assay results to evaluate treatment efficacy, representing the concentration at which 50% inhibition of cell viability was achieved after DOX, gemcitabine, or AMG510 exposure at various time points.
  • Investigating Drug Effects on In Vitro Pancreatic Cancer Cell Models with G12D KRAS Mutation
    Investigating Drug Effects on In Vitro Pancreatic Cancer Cell Models with G12D KRAS Mutation.webp
    This study analyzed the impact of doxorubicin (DOX) and gemcitabine on two in vitro pancreatic cancer cell models carrying the G12D KRAS mutation.IC50 values were derived from ATP assay-based cell viability results and compared between 2D and 3D cultured cells across different time intervals.
Unlocking the Potential of 3D Cultured HepG2 Spheroids for Liver Toxicity Assessment in Drug Development
  • Unlocking the Potential of 3D Cultured HepG2 Spheroids for Liver Toxicity Assessment in Drug Development.webp
  • Experimental Workflow: Assessing Acetaminophen-Induced Toxicity Using 2D and 3D Cultured HepG2 Cells
    Experimental Workflow: Assessing Acetaminophen-Induced Toxicity Using 2D and 3D Cultured HepG2 Cells
  • Morphological Changes in 3D Cultured HepG2 Cells after Repetitive Exposures
    Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures.webp
    Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures.webp
    Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures.webp
    Acetaminophen exposure to 3D HepG2 spheroids resulted in dose-dependent morphological changes, with higher concentrations inducing shrinkage.Quantitative analysis confirmed concentration-dependent inhibition of spheroid growth above 1 mM.
  • Time and Dose-dependent Changes in ATP Viability and IC50 Shift
    Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures.webp
    ATP viability assay showed dose and time-dependent effects, with 0.5 mM initially stimulating growth but declining after 96 hours.
    Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures.webp
    IC50 values decreased with prolonged exposure, emphasizing the importance of extended assessment for cytotoxic evaluation.
  • Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures
    Pattern Shifts in Size and Viability of Long-term Exposed 3D HepG2 Cultures.webp
    Hierarchical clustering analysis revealed time-dependent effects on morphology and viability in 3D HepG2 cultures.Clustering identified distinct separations, particularly at lower to middle doses, and 1 mM exposure aligned with control groups.
  • Positive Correlation between Imaging Data and Viability at Each Time Point
    Positive Correlation between Imaging Data and Viability at Each Time Point.webp
    Correlation analysis between imaging data and viability consistently showed a strong positive relationship at each time point.The significant and reliable correlation between imaging parameters and cellular health confirms their potential as indicators of cellular function.
Conclusion: Long-term repetitive exposure in 3D cultured HepG2 spheroids accurately mirrors the in vivo scenario
  • Long-term repetitive exposure in 3D cultured HepG2 spheroids accurately mirrors the in vivo scenario.webp
    3D HepG2 cells exhibited greater sensitivity to Acetaminophen compared to 2D cells, with significantly lower IC50 values.The close alignment between 3D model's IC50 value and clinical thresholds emphasizes the importance of using physiologically relevant models in toxicity assessments.
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