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Drug Discovery

Biology Research

Our Biology Department boasts a variety of technical solutions, professional scientific research teams, various advanced biological instruments and biological laboratories. Medicilon has rich experience in biological fields such as molecular biology, cell biology, in vitro biology, structural biology, among others.

With our technical platforms such as enzyme level and cell level measurement platforms, we help our clients select compounds and research their properties, action mechanism and bio-marker analysis, thus ensuring the effective implementation of our clients' projects and helping promote the process of drug R&D.

Yeast Protein Expression System
Widely used in the production and preparation of recombinant proteins, especially eukaryotic proteins, the yeast expression system has the advantage of both prokaryotic and advanced eukaryotic systems. It is characterized by common culture conditions, fast growth, low cost, capability of post-translational processing of protein, and easy access to soluble active recombinant protein. Pichia pastoris has become the preferred yeast of choice ffor expressing recombinant proteins due to its rapid growth, abundant commercial expression vectors, and high-efficiency secretory expression.
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E.Coli protein expression system
Prokaryotic expression system (E.coli expression system) is a classical expression system developed earlier and widely used in gene expression technology. In recent decades, E.coli expression system has been continuously developed and improved, and has been widely used by scientific research and industrial users to express various recombinant proteins. Compared with other expression systems, it is characterized by high expression level of target gene, short culture period, strong anti-pollution ability and low cost.
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In Vitro Evaluation of PROTAC Molecules

PROTAC (proteolysis-targeting chimera) is a bifunctional specific small molecule. It can be understood as a dumbbell-shaped chimera, of which one end is used to target the target protein to be degraded, the other end is used to recruit protein degradation systems like E3 ubiquitin ligase and both ends are connected by a suitable linker. Therefore, the chimera is a ternary chimera featuring target protein - PROTAC - E3 ubiquitin ligase that promises close connection between target protein and E3 ligase, so that the target protein can be ubiquitinated and then further recognized and degraded by proteasome.

In theory, this new mechanism can target all drug target proteins, especially those that are previously thought to be undruggable, thus expanding the field of drug research and promising incalculable space and application prospects for new drugs R&D. PROTAC technology is novel and has many advantages compared with traditional micromolecule drugs, antibody drugs, RNAi, etc.. It is considered as one of the popularities in the field of drug R&D.

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Preparation of recombinant kinase
Our Biology Department has rich research experience in the preparation of recombinant kinases. We have various expression systems such as insect cell expression systems, HEK293 cell expression systems and E.coli expression systems. In order to support the selection, discovery and development of kinase drugs, we provide a series of kinase research solutions ranging from detection method development, high-throughput selection to selective analysis. We actively communicate with our clients, understand their specific needs, and provide them with complete experimental technical solutions.
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In Vitro Evaluation of Antibodies

As we know, a complete monoclonal antibody includes an antigen-binding fragment (Fab) and a fragment crystallizable region (Fc). The Fab can bind to a tumor-associated antigen, while the Fc plays an important role in metabolic pathways, as well as in IgG cellular functions.

The molecular sequence and subtype of antibodies will affect the effector functions. Such effector functions include the antibody dependent cellular cytotoxicity (ADCC) triggered by Fc binding to the FcγRIII receptor (CD16A) of NK. The Fc can also bind with serum complement molecules (C1q) to form a membrane attack complex (MAC) that triggers complement-dependent cytotoxicity (CDC). When the Fc binds with macrophage receptors—namely FcγRIII (CD16A), FcγRII (CD32A) and FcγRI (CD64)—the antibody-dependent cellular phagocytosis (ADCP) is triggered.

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