The measurement of the complete three-dimensional structure of a protein and its complex assembly is the scientific basis for studying the relationship between molecular structure and function in life activities, as well as for revealing the physical and chemical nature of life phenomena. X-ray diffraction of protein and its complex crystals is one of the main methods used to study the three-dimensional fine structure of biological macromolecules.
Step | Content | Duration | Remark |
---|---|---|---|
1 | High-throughput-based selection of protein crystals | 4-8 weeks | Commercial selection kit, Mosquito selection |
2 | Diffraction data collection in protein synchrotron radiation | 4-8 weeks | Diffraction data collection in synchrotron radiation for Shanghai |
3 | A. Protein-compound co-crystallization; B. Fragment selection | 4-8 weeks | Compound-binding constant obtained via ITC and Biacore testing recommended |
4 | Analysis of protein crystal | Determined based on specific project | Phase analysis of molecular replacement and heavy metal replacement |