Medicilon Logo
|
search icon search icon language icon contact icon menu icon
Medicilon Logo
|
search icon close search icon language icon contact icon menu icon
CN
Message
Contact Us
Close Button
Back To Top
Online Message×
Click switch
Close Button
X-ray-based Crystal Structural Biology

X-ray-based Crystal Structural Biology

The measurement of complete three-dimensional structure of protein and its complex assembly is the scientific basis for studying the relationship between molecular structure and function in life activities and revealing the physical and chemical nature of life phenomena. The X-ray diffraction of protein and its complex crystals is one of the main methods to study the three-dimensional fine structure of biological macromolecules.

Combining with other disciplines and especially driven bydisciplines like structural genomics, protein crystallography extends from analyzing the simple three-dimensional structure of protein to studying the structure of various biological macromolecules and complexes, to focus on the relationship between structure and function. In pharmaceutical R&D, protein crystallographic structure is widely and significantly used in structure-based novel drug design.
Services we offer
  • High-throughput-based selection of protein crystalsDiffraction data collection in protein synchrotron radiationProtein-compound co-crystallizationAnalysis of protein crystal structure
Items and Steps
Step
Content
Duration
Remark
1High-throughput-based selection of protein crystals4-8 weeksCommercial selection kit, Mosquito selection
2Diffraction data collection in protein synchrotron radiation4-8 weeksDiffraction data collection in synchrotron radiation for Shanghai
3
A. Protein-compound co-crystallization;
B. Fragment selection
4-8 weeksCompound-binding constant obtained via ITC and Biacore testing recommended
4Analysis of protein crystalDetermined based on specific projectPhase analysis of molecular replacement and heavy metal replacement
Remarks
Remarks
You can choose any of the above experiments, or a combination of related experiments, according to your requirements.
Case study-KRAS related assays
  • KRAS-G12D crystallization
    Screening of the co-crystallization.webp
    Screening of KRASG12D
    Synchrotron X-ray diffraction data of the co-crystallization.webp
    Synchrotron X-ray diffraction data of KRASG12D
    Compare the Structure of KRASG12D with 7RPZ.webp
    Compare the Structure of KRASG12D with 7RPZ, the green is PDB ID 7RPZ and the pink is the data from Medicilon.  The data are very correlated.
  • KRAS-G12D-MRTX1133 co-crystallization
    Screening of the co-crystallization.webp
    Screening of the co-crystallization
    Synchrotron X-ray diffraction data of KRASG12D.webp
    Synchrotron X-ray diffraction data of the co-crystallization
    Compare the Structure of KRASG12D co-crystallization with MRTX1133(7RPZ, PDB).webp
    Compare the Structure of KRASG12D co-crystallization with MRTX1133(7RPZ, PDB), the green strucuture is PDB ID 7RPZ and the cyan is data from Medicilon. The data are very correlated.
    Structure of KRASGI2D co-crystallized with MRTX1133 with GDP-bound.webp
    Structure of KRASGI2D co-crystallized with MRTX1133 with GDP-bound