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Enzymology & Molecular Interactions

Enzymology & Molecular Interactions

Our Biology Department has rich experience in the field of in vitro biology, and is available for a complete set of biological services featuring enzyme level measurement, cell level measurement, cell biology, biochemistry, isotope measurement in vitro, establishment of stable cell lines, gene knockout, RNAi and MicroRNA technology, etc.

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Medicilon icon-diamond.pngService
  • In Vitro Enzymology and Molecular Interactions Technology Platform
    Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).
    In-Vitro-Enzymology-and-Molecular-Interactions-Technology-Platform.png
  • Verified biochemical tests
    Validated Enzymatic Targets
    ADTDCKHIF-1aPDGFR beta
    AKT1DNMTsHIF-2aPEP
    AKT2DPPIV/7/8/9HMG-COA reductasePHD1
    AKT3EGFRHSP90PHD2
    ALKEGFR(L858R)Human topoisomerase IPHD3
    ALK(T1151_L1152insTEGFR(L859R,T790M)Human topoisomerase IIPI3K alpha
    ALK(C1156Y)EGFR(L861Q)IDO1PI3K beta
    ALK(F1174L)EGFR(d746-750)IKK betaPI3K gamma
    ALK(L1196M)EGFR(d746-750/T790M)IRAK4PI3K delta
    ALK(G1202R)EGFR(L858R, T790M, C797S)ITKRAC1
    ALK(G1269A)ER-alphaJAK1Renin
    ALK(R1275Q)ER-betaJAK2RET
    ASK1ERK1JAK3RIPK1
    Aurora AERK2KRASROCK1
    Aurora BEPHA2KRAS G12CROCK2
    AXLESR1LCKROR
    Bcl-xlFactor BLipoxygenaseROS
    BLKFactor XaLYNRYR
    BMXFactor XIaMAP4K4(HGK)SAHase
    B-RafFactor IIaMAPK14/p38 alphaSGK1
    BRD4(BD1)FAPMARK1SHP2
    BRD4(BD2)FGFR1MARK2-SIK2SHP2(1-525)
    BTKFGFR2MEK1/MAP2K1SHP2(224-529)
    Cathepsin KFGFR3MNK1SHP2(1-525)_E76K
    Cathepsin LFGFR4MNK2SHP2(1-525)_E69K
    Cathepsin BFlt1/VEGFR1Nectin-4SHP2(1-525)_D61Y
    Cathepsin SFlt3Neutrophil ElastaseSHP2(1-525)_A72V
    DCKFlt4PARP1SPHK1,2
    CDC25BGastric H+/K+ ATPasePARP2SRC
    CDK1Glucosidase alphaPD1/PD-L1Syk
    CDK4Glucosidase betaPDE10A2TDO
    CDK6Glutathione peroxidasePDE1ATEC
    CDK5/P25GSK3 betaPDE2ATXK
    CHK1/CHEK1HDAC1PDE3ATYK2
    CHK2/CHEK2HDAC2PDE4A1Tyrosinase
    Chymase AssayHDAC3PDE4B2TF-FVIIa
    c-KitHDAC4PDE4C1VEGFR2
    c-MetHDAC5PDE4D3Xanthihone oxidase
    COX-1HDAC6PDE520S proteasome
    COX-2HDAC8PDE5A120S Immunoproteasome
    C-peptide RIAHER2PDE6C 
    C-RafHER4PDGFR alpha 
Medicilon icon-diamond.pngMolecular Interactions Platform
As a high-throughput testing system, Biacore 8K can help clients analyze a large number of samples in a short time. Medicilon’s Biology Department is equipped with Biacore 8K and an experienced team that is eager to help clients with their SPR tests.
  • SPR
    SPR, surface plasmon resonance, reveals the interaction between protein and other biomolecules through physical optical effects, and helps clients understand molecular functions and screen effective molecules. SPR tests can replace, and is superior to, tests such as WB/Elisa/CO-IP-/Pull-down/ChIP/EMSA/FRET/yeast two-hybrid tests.
  • Scope of application
    Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
  • Detection indicator
    Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
  • Biacore 8K
    Integration: Integrated solutions from selection to characterization.High throughput: At least 2,300 samples selected in one day, and 64 pairs of kinetic characterization completed in 5 hours.High sensitivity: Even micromolecules or complex targets can result in high-quality data, and the dissociation rate of ultra-high affinity molecules can be accurately characterized.
Relevant laboratories
  • high-speed centrifuge
  • PCR
  • Microplate reader
  • Cultivation room
  • cell room
  • cell crusher
  • pipette
  • Biology-Flow-cytometer
  • BIACORE
  • isotope
  • Molecular Devices1
  • Molecular Devices2
  • Biacore 8K
Relevant articles