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Drug Discovery
Enzymology & Molecular Interactions

Enzymology & Molecular Interactions

Medicilon Biology Department has rich experience in the field of in vitro biology, and offers a complete set of biological services including enzyme level measurement, cell level measurement, cell biology, biochemistry, isotope measurement in vitro, establishment of stable cell lines, gene knockout, RNAi and MicroRNA technology.

ServicesServices
Medicilon icon-diamond.pngService we offer
  • In Vitro Enzymology and Molecular Interactions Technology Platform
    Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).
    In-Vitro-Enzymology-and-Molecular-Interactions-Technology-Platform.png
  • Validated biochemical assays
    CTK:RTK: AGC:CMGC:CAMK:TKL:Atypical:STE:Other:
    BLKALKAKT1CDK1CHK1b-RafPI3K alphaHPK1Aurora A
    BMXAXLAKT2CDK2CHK2c-RafPI3K betaMAP4K4Aurora B
    BTKc-KitAKT3CDK4MARK1IRAK4PI3K gammaMEK1CDC7
    CSKc-MetASK1CDK5/P25MARK2RIPK1DCK
    ITKCSF-1RPKACDK6MNK1RIPK3IKK beta
    JAK1EGFRPKC deltaCDK7MNK2PLK1
    JAK2FGFR1PKC gammaCDK9 WEE1
    JAK3FGFR2

    PKN  A

    CDK12 
    LCKFGFR3

    PKN  B

    ERK1 
    LYNFGFR4

    PKN  C

    ERK2
    SRCFlt1/VEGFR1ROCK1GSK3 beta
    TECFlt3

    ROCK2

    p38 alpha
    TXKFlt4SGK
    TYK2HER2 
    YESHER4
    KDR/VEGFR2
    PDGFR alpha
    PDGFR beta
    RET
    ROS1
    TRKA
    TRKB
    TRKC
    i.e.
    EGFR mutants: EGFR-L858R, EGFR-DEL19, EGFR-DEL19/T790M, EGFR-L858R/T790M, EGFR-DEL19/T790M/C797S, EGFR-L858R/T790M/C797S
    ALK mutants: ALK-G1202R, ALK-L1196M, ALK-F1174L, ALK-C1156Y, ALK-G1269A, ALK-G1269S, ALK-G1202R/L1196M, ALK-T1151-L1152InsT
Medicilon icon-diamond.pngMolecular Interactions Platform
As a high-throughput testing system, Biacore 8K can help clients analyze a large number of samples in a short time. Medicilon’s Biology Department is equipped with Biacore 8K and an experienced team that is eager to help clients with their SPR tests.
  • SPR
    SPR, surface plasmon resonance, reveals the interaction between protein and other biomolecules through physical optical effects, and helps clients understand molecular function and to screen effective molecules. SPR tests can replace and is superior to tests such as WB, Elisa, CO-IP-, Pull-down, ChIP, EMSA, FRET and yeast two-hybrid tests.
  • Scope of application
    Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
  • Detection indicator
    Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
  • Biacore 8K
    Integration: Integrated solutions from selection to characterization.High throughput: At least 2,300 samples selected in one day, and 64 pairs of kinetic characterization completed in 5 hours.High sensitivity: Even micromolecules or complex targets can result in high-quality data, and the dissociation rate of ultra-high affinity molecules can be accurately determined.
  • PDE FamilySPR PlatformProteaseOthers
    PDE1APD-1ThrombinAR
    PDE1BPD-L1FVIIaBcl-xl
    PDE2AVEGFFIXaBRD4
    PDE3ANectin-1FxaCathepsin
    PDE4A1Nectin-2FXIaCox1
    PDE4B2Nectin-3FXIIaCox2
    PDE4C1Nectin-4TrypsinER
    PDE4D3NECL1PlasminFTO
    PDE5A1NECL2TPAHDAC1
    PDE6CNECL3UrokinaseHDAC2
    PDE7ANECL4KallikernHDAC3
    PDE8ANECL5ChymotrypsinHDAC4
    PDE10A1EPHA1TAFIaHDAC5
    EPHA2HDAC6
    EPHA3HDAC8
    EPHA4HIF-1a
    EPHA5HIF-2a
    4-1BBHSP90
    SHP2Kras-WT
    ATIIIKras-G12C
    EGFRKras-G12D
    Gp130PARP1
    OX40PARP2
    TIGITPHD1
    FOLR1PHD2
    INSRPHD3
    IGF-1RPPARα
    HSAPPARγ
    FcRNPPARδ
    FcRIROR
    FcRIIROS
    FcRIIIRYR
    Factor BSHP2
    HER2Tubulin
    TfR1polymerization
    EPCRTyrosinase
    STAT1WRN
    STAT3XO
    STAT5H+K+ATPase
    Enzymatic/Molecular Interaction Screening Platform.webp
Relevant laboratoriesRelevant laboratories
  • high-speed centrifuge
  • PCR
  • Microplate reader
  • Cultivation room
  • cell room
  • cell crusher
  • pipette
  • Biology-Flow-cytometer
  • BIACORE
  •  isotope
  • Molecular Devices1
  • Molecular Devices2
  •  Biacore 8K
Relevant articlesRelevant articles