Enzymology & Molecular Interactions

Our Biology Department has rich experience in the field of in vitro biology, and is available for a complete set of biological services featuring enzyme level measurement, cell level measurement, cell biology, biochemistry, isotope measurement in vitro, establishment of stable cell lines, gene knockout, RNAi and MicroRNA technology, etc.

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In Vitro Enzymology and Molecular Interactions Technology Platform
Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).

Verified biochemical tests

Validated Enzymatic Targets
ADT	DCK	HIF-1a	PDGFR beta
AKT1	DNMTs	HIF-2a	PEP
AKT2	DPPIV/7/8/9	HMG-COA reductase	PHD1
AKT3	EGFR	HSP90	PHD2
ALK	EGFR(L858R)	Human topoisomerase I	PHD3
ALK(T1151_L1152insT	EGFR(L859R,T790M)	Human topoisomerase II	PI3K alpha
ALK(C1156Y)	EGFR(L861Q)	IDO1	PI3K beta
ALK(F1174L)	EGFR(d746-750)	IKK beta	PI3K gamma
ALK(L1196M)	EGFR(d746-750/T790M)	IRAK4	PI3K delta
ALK(G1202R)	EGFR(L858R, T790M, C797S)	ITK	RAC1
ALK(G1269A)	ER-alpha	JAK1	Renin
ALK(R1275Q)	ER-beta	JAK2	RET
ASK1	ERK1	JAK3	RIPK1
Aurora A	ERK2	KRAS	ROCK1
Aurora B	EPHA2	KRAS G12C	ROCK2
AXL	ESR1	LCK	ROR
Bcl-xl	Factor B	Lipoxygenase	ROS
BLK	Factor Xa	LYN	RYR
BMX	Factor XIa	MAP4K4(HGK)	SAHase
B-Raf	Factor IIa	MAPK14/p38 alpha	SGK1
BRD4(BD1)	FAP	MARK1	SHP2
BRD4(BD2)	FGFR1	MARK2-SIK2	SHP2(1-525)
BTK	FGFR2	MEK1/MAP2K1	SHP2(224-529)
Cathepsin K	FGFR3	MNK1	SHP2(1-525)_E76K
Cathepsin L	FGFR4	MNK2	SHP2(1-525)_E69K
Cathepsin B	Flt1/VEGFR1	Nectin-4	SHP2(1-525)_D61Y
Cathepsin S	Flt3	Neutrophil Elastase	SHP2(1-525)_A72V
DCK	Flt4	PARP1	SPHK1,2
CDC25B	Gastric H+/K+ ATPase	PARP2	SRC
CDK1	Glucosidase alpha	PD1/PD-L1	Syk
CDK4	Glucosidase beta	PDE10A2	TDO
CDK6	Glutathione peroxidase	PDE1A	TEC
CDK5/P25	GSK3 beta	PDE2A	TXK
CHK1/CHEK1	HDAC1	PDE3A	TYK2
CHK2/CHEK2	HDAC2	PDE4A1	Tyrosinase
Chymase Assay	HDAC3	PDE4B2	TF-FVIIa
c-Kit	HDAC4	PDE4C1	VEGFR2
c-Met	HDAC5	PDE4D3	Xanthihone oxidase
COX-1	HDAC6	PDE5	20S proteasome
COX-2	HDAC8	PDE5A1	20S Immunoproteasome
C-peptide RIA	HER2	PDE6C
C-Raf	HER4	PDGFR alpha

Molecular Interactions Platform

As a high-throughput testing system, Biacore 8K can help clients analyze a large number of samples in a short time. Medicilon’s Biology Department is equipped with Biacore 8K and an experienced team that is eager to help clients with their SPR tests.

SPR
SPR, surface plasmon resonance, reveals the interaction between protein and other biomolecules through physical optical effects, and helps clients understand molecular functions and screen effective molecules. SPR tests can replace, and is superior to, tests such as WB/Elisa/CO-IP-/Pull-down/ChIP/EMSA/FRET/yeast two-hybrid tests.
Scope of application
Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
Detection indicator
Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
Biacore 8K
Integration: Integrated solutions from selection to characterization.High throughput: At least 2,300 samples selected in one day, and 64 pairs of kinetic characterization completed in 5 hours.High sensitivity: Even micromolecules or complex targets can result in high-quality data, and the dissociation rate of ultra-high affinity molecules can be accurately characterized.

Relevant laboratories

Relevant articles

Computational Biology & Molecular Modeling Enzymology & Molecular Interactions