Enzymology & Molecular Interactions

Medicilon Biology Department has rich experience in the field of in vitro biology, and offers a complete set of biological services including enzyme level measurement, cell level measurement, cell biology, biochemistry, isotope measurement in vitro, establishment of stable cell lines, gene knockout, RNAi and MicroRNA technology.

Services

Coagulation Pathway Studies In Vitro

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Service we offer

In Vitro Enzymology and Molecular Interactions Technology Platform
Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).

Validated biochemical assays

CTK：	RTK：	AGC：	CMGC：	CAMK：	TKL：	Atypical：	STE：	Other：
BLK	ALK	AKT1	CDK1	CHK1	b-Raf	PI3K alpha	HPK1	Aurora A
BMX	AXL	AKT2	CDK2	CHK2	c-Raf	PI3K beta	MAP4K4	Aurora B
BTK	c-Kit	AKT3	CDK4	MARK1	IRAK4	PI3K gamma	MEK1	CDC7
CSK	c-Met	ASK1	CDK5/P25	MARK2	RIPK1			DCK
ITK	CSF-1R	PKA	CDK6	MNK1	RIPK3			IKK beta
JAK1	EGFR	PKC delta	CDK7	MNK2				PLK1
JAK2	FGFR1	PKC gamma	CDK9					WEE1
JAK3	FGFR2	PKN A	CDK12
LCK	FGFR3	PKN B	ERK1
LYN	FGFR4	PKN C	ERK2
SRC	Flt1/VEGFR1	ROCK1	GSK3 beta
TEC	Flt3	ROCK2	p38 alpha
TXK	Flt4	SGK
TYK2	HER2
YES	HER4
	KDR/VEGFR2
	PDGFR alpha
	PDGFR beta
	RET
	ROS1
	TRKA
	TRKB
	TRKC

i.e.
EGFR mutants: EGFR-L858R, EGFR-DEL19, EGFR-DEL19/T790M, EGFR-L858R/T790M, EGFR-DEL19/T790M/C797S, EGFR-L858R/T790M/C797S
ALK mutants: ALK-G1202R, ALK-L1196M, ALK-F1174L, ALK-C1156Y, ALK-G1269A, ALK-G1269S, ALK-G1202R/L1196M, ALK-T1151-L1152InsT

Molecular Interactions Platform

As a high-throughput testing system, Biacore 8K can help clients analyze a large number of samples in a short time. Medicilon’s Biology Department is equipped with Biacore 8K and an experienced team that is eager to help clients with their SPR tests.

SPR
SPR, surface plasmon resonance, reveals the interaction between protein and other biomolecules through physical optical effects, and helps clients understand molecular function and to screen effective molecules. SPR tests can replace and is superior to tests such as WB, Elisa, CO-IP-, Pull-down, ChIP, EMSA, FRET and yeast two-hybrid tests.
Scope of application
Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
Detection indicator
Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
Biacore 8K
Integration: Integrated solutions from selection to characterization.High throughput: At least 2,300 samples selected in one day, and 64 pairs of kinetic characterization completed in 5 hours.High sensitivity: Even micromolecules or complex targets can result in high-quality data, and the dissociation rate of ultra-high affinity molecules can be accurately determined.

PDE Family	SPR Platform	Protease	Others
PDE1A	PD-1	Thrombin	AR
PDE1B	PD-L1	FVIIa	Bcl-xl
PDE2A	VEGF	FIXa	BRD4
PDE3A	Nectin-1	Fxa	Cathepsin
PDE4A1	Nectin-2	FXIa	Cox1
PDE4B2	Nectin-3	FXIIa	Cox2
PDE4C1	Nectin-4	Trypsin	ER
PDE4D3	NECL1	Plasmin	FTO
PDE5A1	NECL2	TPA	HDAC1
PDE6C	NECL3	Urokinase	HDAC2
PDE7A	NECL4	Kallikern	HDAC3
PDE8A	NECL5	Chymotrypsin	HDAC4
PDE10A1	EPHA1	TAFIa	HDAC5
	EPHA2		HDAC6
	EPHA3		HDAC8
	EPHA4		HIF-1a
	EPHA5		HIF-2a
	4-1BB		HSP90
	SHP2		Kras-WT
	ATIII		Kras-G12C
	EGFR		Kras-G12D
	Gp130		PARP1
	OX40		PARP2
	TIGIT		PHD1
	FOLR1		PHD2
	INSR		PHD3
	IGF-1R		PPARα
	HSA		PPARγ
	FcRN		PPARδ
	FcRI		ROR
	FcRII		ROS
	FcRIII		RYR
	Factor B		SHP2
	HER2		Tubulin
	TfR1		polymerization
	EPCR		Tyrosinase
	STAT1		WRN
	STAT3		XO
	STAT5		H+K+ATPase

Enzymatic/Molecular Interaction Screening Platform.webp

Relevant laboratories

Relevant articles

Coagulation Pathway Studies In Vitro