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Drug Discovery
Enzymology & Molecular Interactions

Enzymology & Molecular Interactions

Medicilon Biology Department has rich experience in the field of in vitro biology, and offers a complete set of biological services including enzyme level measurement, cell level measurement, cell biology, biochemistry, isotope measurement in vitro, establishment of stable cell lines, gene knockout, RNAi and MicroRNA technology.

ServicesServices
Medicilon icon-diamond.pngService we offer
  • In Vitro Enzymology and Molecular Interactions Technology Platform
    Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).
    In-Vitro-Enzymology-and-Molecular-Interactions-Technology-Platform.png
  • Validated biochemical assays
    Enzymatic/Molecular Interaction Screening Platform
    PDE FamilyProteaseOthers
    PDE1AThrombinAR
    PDE1BFVIIaBcl-xl
    PDE2AFIXaBRD4
    PDE3AFxaCathepsin
    PDE4A1FXIaCox1
    PDE4B2FXIIaCox2
    PDE4C1TrypsinER
    PDE4D3PlasminFTO
    PDE5A1TPAHDAC1
    PDE6CUrokinaseHDAC2
    PDE7AKallikernHDAC3
    PDE8AChymotrypsinHDAC4
    PDE10A1TAFIaHDAC5
    HDAC6
    HDAC8
    HIF-1a
    HIF-2a
    HSP90
    Kras-WT
    Kras-G12C
    Kras-G12D
    PARP1
    PARP2
    PHD1
    PHD2
    PHD3
    PPARα
    PPARγ
    PPARδ
    ROR
    ROS
    RYR
    SHP2
    Tubulin
    polymerization
    Tyrosinase
    WRN
    XO
    H+K+ATPase
Medicilon icon-diamond.pngMolecular Interactions Platform
As a high-throughput testing system, Biacore 8K can help clients analyze a large number of samples in a short time. Medicilon’s Biology Department is equipped with Biacore 8K and an experienced team that is eager to help clients with their SPR tests.
  • SPR
    SPR, surface plasmon resonance, reveals the interaction between protein and other biomolecules through physical optical effects, and helps clients understand molecular function and to screen effective molecules. SPR tests can replace and is superior to tests such as WB, Elisa, CO-IP-, Pull-down, ChIP, EMSA, FRET and yeast two-hybrid tests.
  • Scope of application
    Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
  • Detection indicator
    Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
  • Biacore 8K
    Integration: Integrated solutions from selection to characterization.High throughput: At least 2,300 samples selected in one day, and 64 pairs of kinetic characterization completed in 5 hours.High sensitivity: Even micromolecules or complex targets can result in high-quality data, and the dissociation rate of ultra-high affinity molecules can be accurately determined.
    PD-1EPHA2IGF-1RPD-L1EPHA3HSAVEGFEPHA4FcRNNectin-1EPHA5FcRINectin-24-1BBFcRIINectin-3SHP2FcRIIINectin-4ATIIIFactor BNECL1EGFRHER2NECL2Gp130TfR1NECL3OX40EPCRNECL4TIGITSTAT1NECL5FOLR1STAT3EPHA1INSRSTAT5
    Enzymatic/Molecular Interaction Screening Platform.webp            Enzymatic/Molecular Interaction Screening Platform.webp
    Figure 1. Nivolumab binding with PD-1
    Figure 2. Novoseven binding with TF
    Figure 3. EFNA1 binding with EphA2
    Figure 4. Novoseven binding with ATIII
    Validated targets:PD-1, PD-L1, VEGF, Nectin1, Nectin2, Nectin3, Nectin4, NECL1, NECL2, NECL3, NECL4, NECL5, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, INSR, IGF-1R, HSA, FcRN, FcRI, FcRII, FcRIII, C1q, Factor B, HER2, Transferrin, EPCR, STAT3, STAT5, STAT1, 4-1BB, SHP2, ATIII, EGFR, gp130
Relevant laboratoriesRelevant laboratories
  • high-speed centrifuge
  • PCR
  • Microplate reader
  • Cultivation room
  • cell room
  • cell crusher
  • pipette
  • Biology-Flow-cytometer
  • BIACORE
  •  isotope
  • Molecular Devices1
  • Molecular Devices2
  •  Biacore 8K
Relevant articlesRelevant articles