Enzymology & Molecular Interactions

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• In Vitro Enzymology and Molecular Interactions Technology Platform
  Fluorescence analysis methods: Lanthascreen; Alphascreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).
  
• Verified biochemical tests

  Validated Enzymatic Targets
  ADT	DCK	HIF-1a	PDGFR beta
  AKT1	DNMTs	HIF-2a	PEP
  AKT2	DPPIV/7/8/9	HMG-COA reductase	PHD1
  AKT3	EGFR	HSP90	PHD2
  ALK	EGFR(L858R)	Human topoisomerase I	PHD3
  ALK(T1151_L1152insT	EGFR(L859R,T790M)	Human topoisomerase II	PI3K alpha
  ALK(C1156Y)	EGFR(L861Q)	IDO1	PI3K beta
  ALK(F1174L)	EGFR(d746-750)	IKK beta	PI3K gamma
  ALK(L1196M)	EGFR(d746-750/T790M)	IRAK4	PI3K delta
  ALK(G1202R)	EGFR(L858R, T790M, C797S)	ITK	RAC1
  ALK(G1269A)	ER-alpha	JAK1	Renin
  ALK(R1275Q)	ER-beta	JAK2	RET
  ASK1	ERK1	JAK3	RIPK1
  Aurora A	ERK2	KRAS	ROCK1
  Aurora B	EPHA2	KRAS G12C	ROCK2
  AXL	ESR1	LCK	ROR
  Bcl-xl	Factor B	Lipoxygenase	ROS
  BLK	Factor Xa	LYN	RYR
  BMX	Factor XIa	MAP4K4(HGK)	SAHase
  B-Raf	Factor IIa	MAPK14/p38 alpha	SGK1
  BRD4(BD1)	FAP	MARK1	SHP2
  BRD4(BD2)	FGFR1	MARK2-SIK2	SHP2(1-525)
  BTK	FGFR2	MEK1/MAP2K1	SHP2(224-529)
  Cathepsin K	FGFR3	MNK1	SHP2(1-525)_E76K
  Cathepsin L	FGFR4	MNK2	SHP2(1-525)_E69K
  Cathepsin B	Flt1/VEGFR1	Nectin-4	SHP2(1-525)_D61Y
  Cathepsin S	Flt3	Neutrophil Elastase	SHP2(1-525)_A72V
  DCK	Flt4	PARP1	SPHK1,2
  CDC25B	Gastric H+/K+ ATPase	PARP2	SRC
  CDK1	Glucosidase alpha	PD1/PD-L1	Syk
  CDK4	Glucosidase beta	PDE10A2	TDO
  CDK6	Glutathione peroxidase	PDE1A	TEC
  CDK5/P25	GSK3 beta	PDE2A	TXK
  CHK1/CHEK1	HDAC1	PDE3A	TYK2
  CHK2/CHEK2	HDAC2	PDE4A1	Tyrosinase
  Chymase Assay	HDAC3	PDE4B2	TF-FVIIa
  c-Kit	HDAC4	PDE4C1	VEGFR2
  c-Met	HDAC5	PDE4D3	Xanthihone oxidase
  COX-1	HDAC6	PDE5	20S proteasome
  COX-2	HDAC8	PDE5A1	20S Immunoproteasome
  C-peptide RIA	HER2	PDE6C
  C-Raf	HER4	PDGFR alpha

Molecular Interactions Platform

• SPR
  SPR, surface plasmon resonance, reveals the interaction between protein and other biomolecules through physical optical effects, and helps clients understand molecular functions and screen effective molecules. SPR tests can replace, and is superior to, tests such as WB/Elisa/CO-IP-/Pull-down/ChIP/EMSA/FRET/yeast two-hybrid tests.
• Scope of application
  Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
• Detection indicator
  Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
• Biacore 8K
  Integration: Integrated solutions from selection to characterization.High throughput: At least 2,300 samples selected in one day, and 64 pairs of kinetic characterization completed in 5 hours.High sensitivity: Even micromolecules or complex targets can result in high-quality data, and the dissociation rate of ultra-high affinity molecules can be accurately characterized.

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