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CHO Cell Protein Expression System

Featuring protein folding and post-translational modification, mammalian protein expression systems can make proteins closer to the natural ones, thus obtaining the same biological activity as natural proteins. Therefore, the mammalian cell expression system has been widely applied in the R&D and production of recombinant protein drugs, especially therapeutic recombinant monoclonal antibody drugs.

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Services

Based on a serum-free culture system, our mammalian protein expression system offers various eukaryotic protein services, including gene expression optimization, transient expression, stable expression, and antibody production.

Our researchers have established a mature mammalian protein expression service platform to provide expression and purification of mammalian proteins.

Mammalian protein expression system services
Construction and preparation of recombinant mammalian cell expression vectorScreening of transient expression of recombinant protein or antibody in CHO and HEK293 cellsConstruction of recombinant protein or antibody-stabilized cell lines (CHO-S and CHO-DG44 cell platforms) CHO suspension cell in bioreactor (3-30L)Culture of hybridoma cells in a roller bottle or bioreactor

Items and Steps

Step	Content	Duration	Remark
1	Construction and preparation of recombinant mammalian cell expression vector	3 weeks	Use of different tags: Fc, Human Serum Albumin, 3xFlag, GST, 6His, etc.
2	Transient expression test of proteins or antibodies in CHO and HEK293 cells	2 weeks	Western Blot assay
3	Transient expression of recombinant proteins or antibodies in large volumes	4 week	PEI transient transfection
4	Construction of recombinant protein or antibody-stabilized cell lines	TBD	CHO-S and CHO-DG44 cell systems
5	Culture of CHO suspension cell bioreactor	TBD	3-30L

Rabbit IgG expressed in transient transfected 293 cells

Remarks

the condition medium was run in 10%SDS-PAGE gel and stained with coomassie blue dye.

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