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Drug Discovery

CHO Cell Protein Expression System

Featuring protein folding and post-translational modification, mammalian protein expression systems can make proteins closer to the natural ones, thus obtaining the same biological activity as natural proteins. Therefore, the mammalian cell expression system has been widely applied in the R&D and production of recombinant protein drugs, especially therapeutic recombinant monoclonal antibody drugs.
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Medicilon icon-diamond.pngServices
Based on a serum-free culture system, our mammalian protein expression system offers various eukaryotic protein services, including gene expression optimization, transient expression, stable expression, and antibody production.
Our researchers have established a mature mammalian protein expression service platform to provide expression and purification of mammalian proteins.
  • Mammalian protein expression system services
    Construction and preparation of recombinant mammalian cell expression vectorScreening of transient expression of recombinant protein or antibody in CHO and HEK293 cellsConstruction of recombinant protein or antibody-stabilized cell lines (CHO-S and CHO-DG44 cell platforms) CHO suspension cell in bioreactor (3-30L)Culture of hybridoma cells in a roller bottle or bioreactor
Medicilon icon-notebook.pngItems and Steps
StepContentDurationRemark
1Construction and preparation of recombinant mammalian cell expression vector3 weeksUse of different tags: Fc, Human Serum Albumin, 3xFlag, GST, 6His, etc.
2Transient expression test of proteins or antibodies in CHO and HEK293 cells2 weeksWestern Blot assay
3Transient expression of recombinant proteins or antibodies in large volumes4 weekPEI transient transfection
4Construction of recombinant protein or antibody-stabilized cell linesTBDCHO-S and CHO-DG44 cell systems
5Culture of CHO suspension cell bioreactorTBD3-30L
Rabbit IgG expressed in transient transfected 293 cells
Rabbit IgG expressed in transient transfected 293 cells.png
Remarks

the condition medium was run in 10%SDS-PAGE gel and stained with coomassie blue dye.

Relevant laboratories
  • high-speed centrifuge
  • Hamilton-liquid-handling-system
  • 培养室
  • AKTA-xpress purification system
  • cell room
  • cell crusher
  • Cell Spinner Flask Incubator
  • Biology laboratory
  • pipette
Relevant articles