Protein Expression and Purification

We have rich experience in the expression and purification of recombinant proteins. We are proud of our various protein expression systems, including our prokaryotic protein expression system, yeast protein expression system, insect cell protein expression system (baculovirus), and mammalian cell protein expression system. In addition, we have employed fusion technologies to provide our clients with more options in terms of protein expression and purification. We tailor our service for your project at different dimensions including the scheme design, gene optimization, expression condition optimization, and purification technology system of your target protein expression level.

Services

Protein Purification
We provide affinity chromatography purification for tag protein, which purifies the expressed recombinant protein by means of molecular exclusion chromatography (molecular sieve), ion exchange chromatography, hydrophobic chromatography, and gel filtration, to remove the unnecessary purified tags.
Molecular exclusion chromatography (molecular sieve)Ion exchange chromatography (IEX)Hydrophobic chromatography Gel filtration
Customized protein purification Protein purification is an important step. Choosing an appropriate method is helpful to obtain better protein products. As for the choice of methodology, you can make your request and discuss with us. The purified protein can be used in follow-up experiments such as compound screening, activity detection, structure research, antibody preparation, ICH, cell experiment, protein interaction research and drug target research. Flexible selection of protein purification tags The selection of protein expression purification tags will have a certain impact on protein expression, solubility, and the follow-up application of protein. Medicilon can offer flexible tag selection (tags such as his, GAT, MBP and sumo)
Preparation of recombinant kinase
Various expression systems: insect cell, HEK293 cell and E.coliHoloenzyme or kinase Inhibitor selection level or structural biological level