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Drug Discovery
Protein Expression and Purification

Protein Expression and Purification

We have significant experience in the expression and purification of recombinant proteins. We utilise various protein expression modalities, including prokaryotic protein expression, yeast protein expression, insect cell protein expression (baculovirus), and mammalian cell protein expression systems. In addition, we have employed fusion technologies to provide our clients with more options in terms of protein expression and purification. We tailor our service for your project including the design, gene optimization, expression condition optimization, and purification technology system to deliver your target protein quality and expression level.



ServicesServices

  • Protein Purification
    We provide affinity chromatography purification for tag protein, which purifies the expressed recombinant protein by means of molecular exclusion chromatography, ion exchange chromatography, hydrophobic chromatography, and gel filtration, to remove the unnecessary purified tags.
    Molecular exclusion chromatography (molecular sieve)Ion exchange chromatography (IEX)Hydrophobic chromatography Gel filtration
    Customized protein purification Protein purification is a critical step. Choosing an appropriate method is essential to obtain superior protein products andr the choice of methodology, can be selected in discussion with our expert team. The purified protein can be used in follow-up experiments such as compound screening, activity detection, structure research, antibody preparation, ICH, cell experiment, protein interaction research and drug target research. Flexible selection of protein purification tags  The selection of protein expression purification tags will have an impact on protein expression, solubility, and the follow-up application of protein. Medicilon can offer flexible tag selection including his, GAT, MBP and sumo options.
  • Preparation of recombinant kinase
    Various expression systems: insect cell, HEK293 cell and E.coliHoloenzyme or kinase Inhibitor selection level or structural biological level
Relevant laboratoriesRelevant laboratories
  • Biology lab
  • AKTA-press Purification system
  • High speed centrifuge
  • bioreactor
  • chromatograph
  • Cell crusher
  • Cell chamber
  • Pipetting station
  • pipette
  • Overspeed centrifuge
  • Protein Purification instrument AKTA Pure 25
  • Protein Purification instrument
  • Automated pipetting workstation
Relevant articlesRelevant articles