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Drug Discovery

Yeast Protein Expression System

Widely used in the production and preparation of recombinant proteins, especially eukaryotic proteins, the yeast expression system has the advantage of both prokaryotic and advanced eukaryotic systems. It is characterized by common culture conditions, fast growth, low cost, capability of post-translational processing of protein, and easy access to soluble active recombinant protein. Pichia pastoris has become the preferred yeast of choice ffor expressing recombinant proteins due to its rapid growth, abundant commercial expression vectors, and high-efficiency secretory expression.
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Medicilon icon-diamond.pngServices
Our researchers have established a mature yeast expression service platform to provide expression and purification of quality recombinant proteins in pichia pastoris.


  • Yeast expression system services:
    Construction of recombinant yeast expression plasmidElectrotransformation of recombinant plasmidRecombinant expressor screeningExpression and purification of small-scale proteins (product cultured in 2L medium);Expression and purification of large-scale proteins (over 10L)
Medicilon icon-notebook.pngItems and Steps
StepContentDurationRemark
1Construction of recombinant yeast expression plasmid2 weeksDifferent carriers available; different affinity tags; secretion or intracellular expression
2Electrotransformation of recombinant plasmid1 weekDifferent expression bacterial strains available; 5-10 clones for PCR identification
3Screening of recombinant expressors3-4 weeks5-10 clones for screening, recombinants screening based on Zeocin antibiotic concentration, and identification of recombinant protein expressors by Western blot assays
4Expression and purification of small-scale proteins2-4 weeksOptimize culture medium, cell density, methanol concentration, induction time, etc.
5Expression and purification of large-scale proteinsDetermined based on the expressed volumeShake flask or culture fermentor available
Remarks

You can choose any of the above experiments, or a combination of related experiments, according to your requirements.

Affinity purification, ion column purification, hydrophobic packing purification, or molecular sieve purification are available for the purification options.

The nature of proteins, as well as the expression system selected, will affect the purity and activity of the final products.

Relevant laboratories
  • AKTA-xpress purification system
  • Hamilton-liquid-handling-system
  • high-speed centrifuge
  • Cultivation room
  • Bioreactor
  • cell crusher
  • Cell Spinner Flask Incubator
  • microscope
  • pipetting workstation
Relevant articles