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Jan 04,2022
Types of Ligand Binding Assays
After ingesting biotechnology drugs, the body will produce different degrees of immune response based on the immunogenicity of the drug. The drug stimulates the body to form specific anti-drug antibodies (ADA) for the immunogenicity of the drug, allergies, autoimmunity and different The pharmacokinetic characteristics of the drug are anti-drug antibody reactions, which can cause variousRead more
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Nov 24,2021
ELISA Protocol-Elisa Experiment Standard Operating Method
The Elisa experiment has been widely used clinically due to its high sensitivity and good specificity. However, each link in the operation has a greater impact on the detection effect of the experiment. If you are not careful, it may lead to incomplete color rendering and patterning. Wait for the result. ELISA Protocol The followingRead more
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Nov 24,2021
Disadvantages of Indirect ELISA
Enzyme linked immunosorbent assay (ELISA) refers to the binding of antigen or antibody to the surface of a solid-phase carrier and maintain its immunological activity. The antigen or antibody is linked with a certain enzyme to form an enzyme-labeled antigen or antibody. This enzyme-labeled antigen or antibody retains its immune activity and enzyme activity. CommonRead more
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Nov 04,2021
Influence of Hemolysis on Test Results
Specimen hemolysis is one of the most common interference and influence factors in clinical biochemical examination. Hemolysis leads to inaccurate test results, which cannot objectively reflect the patient’s physical condition at that time. The influence of specimen hemolysis on clinical test results is as follows: 1. Influence on RBC Count and RBC Specific Volume  In hemolysisRead more
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Nov 03,2021
Abnormal Result Caused by Hemolysis of Blood Samples
⑴ Influence on biochemical results: Specimen hemolysis is the most common interference and influencing factor in clinical biochemical tests. Hemolysis has an impact on a considerable part of biochemical testing items. Among them, total bilirubin (TBIL), aspartate aminotransferase (AST), total protein (TP), and albumin (ALB) are significantly increased after hemolysis; significant after hemolysis The itemsRead more
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Nov 03,2021
Influence of Hemolysis on the Analysis of Biological Samples
Hemolysis refers to the rupture of red blood cells and the escape of hemoglobin into plasma or serum, which can occur in vivo and in vitro. The factors affecting hemolysis are also very complicated. After hemolysis of plasma and serum, many physiological components such as hemoglobin, bilirubin, phospholipids, etc. increase significantly. These components may affectRead more
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Nov 03,2021
Hemolysis Test
Hemolysis test: refers to the hemolysis and red blood cell aggregation caused by pharmaceutical preparations. Hemolytic reactions include immune hemolysis and non-immune hemolysis. 1. Prepare 2% Red Blood Cells Baoding rabbits, draw blood from the heart 5 m, add 0.2 ml of anticoagulant EDTA, wash with PBS, centrifuge to remove the white blood cells onRead more
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Sep 23,2021
hERG and its Evaluation in Drug Development
Drug safety evaluation plays an extremely important role in the drug development process. As some non-cardiovascular drugs have been found to induce acquired QT prolonged syndrome (LQTS), leading to severe arrhythmia (Torsade de Pointes) , TdP) and after withdrawing from the market, the safety evaluation of the drug on the heart becomes extremely important. TheRead more
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Sep 23,2021
Common ELISA Format
The competitive ELISA format is based on the binding competition in the initial insulin RIA and IgG EIA methods. The competition between the target analyte from the sample and the reference analyte and the binding of the analyte-specific antibody is the key to this format. Either antibody or target analyte can be labeled with enzymeRead more
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Sep 22,2021
Direct vs Indirect Elisa
All you need to know about the ELISA experiment is here. ELISA, the full name of enzyme-linked immunosorbent assay, is a commonly used immunoassay method; Detection Principle The known antigen or antibody is adsorbed on the surface of the solid phase carrier, the antigen or antibody is labeled with enzyme, and the antigen or antibodyRead more
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Aug 19,2021
How to do a good job of biological analysis of peptide drugs?
Since Robert Bruce Merrifield synthesized the first biologically active peptide in 1953, research on peptide drugs has developed rapidly, and research on peptide drugs targeting multiple receptors has even been carried out internationally. The maturity of peptide synthesis technology has further accelerated the development of peptide drugs and has become one of the international researchRead more
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Aug 18,2021
Do you know about western blotting?
IBT and LIA The Western blot detection technology currently used is not the original Western blot (IBT) but an optimized and improved method. western blotting Traditional Western Blotting (IBT): the protein is separated and purified by electrophoresis and transferred to a nitrocellulose membrane, and then autoimmune serum is used as a probe to react withRead more
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Aug 04,2021
The experimental principle and operation steps of ELISA
1. Experimental principle ELISA is a highly sensitive test technique based on immunological reaction, which combines the specific reaction of antigen and antibody with the efficient catalysis of enzyme to substrate. Since the reaction of antigen and antibody is carried out in a solid phase carrier — polystyrene microtitration plate, after each addition of aRead more
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Aug 04,2021
ELISA Experiment Guide: Indirect ELISA
Indirect ELISA Indirect ELISA is illustrated in the figure below and Figure 2. Phases I and II are similar to the direct system.Phase III involves adding unlabeled detection antibodies that are diluted in the buffer to prevent the proteins in the antiserum from nonspecifically attaching to the solid phase (the blocking buffer). It is thenRead more
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Aug 03,2021
ELISA Indirect Method
As we all know, enzyme-linked immunosorbent assay (ELISA) is a technique that uses antigen-antibody interaction to quantify proteins, hormones, antibodies or viral antigens. Up to now, more than 200 research papers related to ELISA and antibody detection have been published in journals around the world every year. Among them, there are more than 70 papersRead more
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Aug 03,2021
Indirect ELISA procedure
Indirect ELISA procedure-Experimental materials 1. 96-well microplate 2. TMB Experimental reagents 1. Encapsulation solution (pH9.6) : Na2CO3 0.17g, NaHCO3 0.28g, add distilled water to 100ml.4 ℃. 2. Washing solution (PBST): KH2PO4 0.2g, KCl 0.2g, NaCl 8.0g, Na2HPO4·12H2O 2.9g, Tween-20 0.5mL, add deionized water to 1000mL, adjust pH to 7.2-7.4. 3. Substrate reaction liquid: (1) SubstrateRead more
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Jul 31,2021
Bioanalytical Research Method
With the rapid development of the pharmaceutical industry, the research of biotechnology drugs has also entered a higher level of platform. Biotechnology drugs refer to therapeutic drugs produced through recombinant DNA technology or other innovative biotechnology-based biotechnology, which mainly include cytokines, plasminogen activators, recombinant plasma factors, growth factors, antibodies, vaccines and oligonucleotide drugs It isRead more
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Jul 13,2021
Aloe-emodin inhibits tumor cell activity
Anthraquinone compounds are one of the important active components of aloe, which mainly include aloe emodin, chrysophanol, emodin, rhein acid and emodin methyl ether. A large number of cell experiments have shown that the anthraquinone compound aloe emodin can inhibit tumor cell proliferation and induce tumor cell apoptosis by blocking cell cycle. Therefore, the researchRead more
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Apr 02,2021
Details of success or failure in ELISA experiment operation
Enzyme-linked immunosorbent assay (ELISA) is widely used in the determination of various antigens and antibodies in clinical testing because of its high sensitivity and strong specificity. Although the ELISA operation is simple, there are also big articles in the small experiment. Each link of the ELISA operation has a great influence on the detection effectRead more
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Apr 02,2021
Influencing factors of wrong results of ELISA
Influencing factors of wrong results of ELISA ELISA, or enzyme-linked adsorption test, is a commonly used solid-phase enzyme immunoassay method. Engvall and Perlmann first applied this method to quantify IgG in 1971, and named it “enzyme linked immunosorbent assay (ELISA)“. ELISA The basic principle of ELISA is: ① Make the antigen (or antibody) bind toRead more
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