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Bioanalysis is an important part of the drug development process, including pharmacokinetics (PK) and biomarker analysis, which runs through the entire process of drug development from early screening to clinical research to post-marketing monitoring. Pharmacokinetics is usually an important end point of clinical research. Non-clinical and clinical pharmacokinetics not only guide the dosage selection ofRead more

The biological analysis described in this article is to determine the concentration of biological drugs in animal and human body fluids or tissues (this article specifically refers to protein biological drugs, including monoclonal antibodies, cytokines, auxins, fusion proteins, etc.). Most bioanalytical methods are based on immunoassays (Immunoassays), or more broadly called, ligand binding assays (LBA).Read more

In the previous issues, I shared the past and present of the matrix effect of small molecule LC-MS/MS bioanalysis with friends in the circle. This issue returns to the topic of macromolecular bioanalysis. Ligand Binding Assay (LBA) is the most widely used detection method for macromolecular bioanalysis. LBA is based on the equilibrium reaction betweenRead more

There are numerous types of ligand binding assays, both radioactive and non-radioactive.  As such, ligand binding assays are a superset of radio-binding assays, which are the conceptual inverse of radioimmunoassays (RIA). Some newer types are called “mix-and-measure” assays because they do not require separation of bound ligands. Ligand binding assays are used primarily in pharmacologyRead more