Containligand-binding-assayContent

After ingesting biotechnology drugs, the body will produce different degrees of immune response based on the immunogenicity of the drug. The drug stimulates the body to form specific anti-drug antibodies (ADA) for the immunogenicity of the drug, allergies, autoimmunity and different The pharmacokinetic characteristics of the drug are anti-drug antibody reactions, which can cause variousRead more

Specimen hemolysis is one of the most common interference and influence factors in clinical biochemical examination. Hemolysis leads to inaccurate test results, which cannot objectively reflect the patient’s physical condition at that time. The influence of specimen hemolysis on clinical test results is as follows: 1. Influence on RBC Count and RBC Specific Volume  In hemolysisRead more

⑴ Influence on biochemical results: Specimen hemolysis is the most common interference and influencing factor in clinical biochemical tests. Hemolysis has an impact on a considerable part of biochemical testing items. Among them, total bilirubin (TBIL), aspartate aminotransferase (AST), total protein (TP), and albumin (ALB) are significantly increased after hemolysis; significant after hemolysis The itemsRead more

Hemolysis refers to the rupture of red blood cells and the escape of hemoglobin into plasma or serum, which can occur in vivo and in vitro. The factors affecting hemolysis are also very complicated. After hemolysis of plasma and serum, many physiological components such as hemoglobin, bilirubin, phospholipids, etc. increase significantly. These components may affectRead more

Hemolysis test: refers to the hemolysis and red blood cell aggregation caused by pharmaceutical preparations. Hemolytic reactions include immune hemolysis and non-immune hemolysis. 1. Prepare 2% Red Blood Cells Baoding rabbits, draw blood from the heart 5 m, add 0.2 ml of anticoagulant EDTA, wash with PBS, centrifuge to remove the white blood cells onRead more

Drug safety evaluation plays an extremely important role in the drug development process. As some non-cardiovascular drugs have been found to induce acquired QT prolonged syndrome (LQTS), leading to severe arrhythmia (Torsade de Pointes) , TdP) and after withdrawing from the market, the safety evaluation of the drug on the heart becomes extremely important. TheRead more

NM23 is a tumor metastasis suppressor gene, which has regulatory functions on cell proliferation, differentiation and ontogeny. At present, 10 subtypes of NM23 have been found, and the more thorough research is NM23A and NM23B. Western blot analysis shows that NM23B has hepatocyte proliferation effect on SD rats cultured in vitro. Western blot analysis, alsoRead more

IBT and LIA The Western blot detection technology currently used is not the original Western blot (IBT) but an optimized and improved method. western blotting Traditional Western Blotting (IBT): the protein is separated and purified by electrophoresis and transferred to a nitrocellulose membrane, and then autoimmune serum is used as a probe to react withRead more

The Biology Department of Shanghai Medicilon has extensive experience in the field of in vitro biology, through enzyme level determination, cell level determination, cell biology, biochemistry, in vitro isotope determination, stable cell line establishment, gene knockout, RNAi and MicroRNA Technology, etc., to provide a complete set of biological services. In cell biology research, sometimes itRead more

Biological analysis refers to the quantitative analysis of target substances in biological matrix by chromatography, chromatography-mass spectrometry, ligand binding and other techniques. Bioanalysis includes the analysis of drugs, metabolites, proteins, peptides and other large and small molecules in the matrix. In the pharmaceutical industry, bioanalysis has been widely used in the process of drug discoveryRead more

1. Experimental principle ELISA is a highly sensitive test technique based on immunological reaction, which combines the specific reaction of antigen and antibody with the efficient catalysis of enzyme to substrate. Since the reaction of antigen and antibody is carried out in a solid phase carrier — polystyrene microtitration plate, after each addition of aRead more

As we all know, enzyme-linked immunosorbent assay (ELISA) is a technique that uses antigen-antibody interaction to quantify proteins, hormones, antibodies or viral antigens. Up to now, more than 200 research papers related to ELISA and antibody detection have been published in journals around the world every year. Among them, there are more than 70 papersRead more

Indirect ELISA procedure-Experimental materials 1. 96-well microplate 2. TMB Experimental reagents 1. Encapsulation solution (pH9.6) : Na2CO3 0.17g, NaHCO3 0.28g, add distilled water to 100ml.4 ℃. 2. Washing solution (PBST): KH2PO4 0.2g, KCl 0.2g, NaCl 8.0g, Na2HPO4·12H2O 2.9g, Tween-20 0.5mL, add deionized water to 1000mL, adjust pH to 7.2-7.4. 3. Substrate reaction liquid: (1) SubstrateRead more

With the rapid development of the pharmaceutical industry, the research of biotechnology drugs has also entered a higher level of platform. Biotechnology drugs refer to therapeutic drugs produced through recombinant DNA technology or other innovative biotechnology-based biotechnology, which mainly include cytokines, plasminogen activators, recombinant plasma factors, growth factors, antibodies, vaccines and oligonucleotide drugs It isRead more

The acquisition of lead compounds and the production of drugs are important links in the process of drug screening. Drug screening involves a series of steps to select the most suitable chemical entity for drug development to clinical application, including pharmacological research and toxicological research. Research to determine the safety and effectiveness of the drug.Read more

Anthraquinone compounds are one of the important active components of aloe, which mainly include aloe emodin, chrysophanol, emodin, rhein acid and emodin methyl ether. A large number of cell experiments have shown that the anthraquinone compound aloe emodin can inhibit tumor cell proliferation and induce tumor cell apoptosis by blocking cell cycle. Therefore, the researchRead more

CC chemokine 2 (CCL2), which is generally expressed in white blood cells and rarely expressed in normal nerve tissues, plays an important role in the innate immunity caused by tissue damage and some diseases. Numerous studies have found that CCL2 can damage the learning and memory of rats. In order to study the effect andRead more

PROTAC technology has a unique mechanism The PROTAC molecule specifically recognizes and binds to the target protein through the target protein ligand (POI Ligand) at one end and the E3 Ligase through the E3 Ubiquitin Ligase Ligand (E3 Ligase Ligand) at the other end.Forming the POI-PROTAC-E3 Ligase Ternary ComplexIn this ternary complex, the target proteinRead more

The principle of yeast two-hybrid system The yeast transcriptional activator GAL4 is composed of two separable and functionally independent domains. There is a DNA binding domain (BD) at the N terminal, which consists of 147 amino acids.There is a transcription activation domain (AD) at C terminal, which consists of 113 amino acids.BD can be combinedRead more

Enzyme-linked immunosorbent assay (ELISA) is widely used in the determination of various antigens and antibodies in clinical testing because of its high sensitivity and strong specificity. Although the ELISA operation is simple, there are also big articles in the small experiment. Each link of the ELISA operation has a great influence on the detection effectRead more