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Jan 04,2022
Sincere Collaboration, New and Far-Reaching - Bao Pharmaceutical and Medicilon reached a strategic collaboration
On December 17, 2021, Shanghai Bao Pharmaceutical Co., Ltd. (Bao) and Shanghai Medicilon Inc. (Medicilon) signed an innovative drug IND R&D service strategy collaboration agreement.
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Nov 29,2021
SeMet-labeled Protein High-yield Expression Medium
Selenium has been recognized by the World Health Organization as an essential trace element for the human body. It has strong anti-tumor and immunity enhancement effects. Compared with inorganic selenium, organic selenium has low toxicity, high bioavailability, and strong pharmacological activity. Selenomethionine is a natural organic selenium product with very few sources. Therefore, preparation byRead more
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Oct 28,2021
Application of Yeast Expression System
Background Yeast expression system is the most economical and efficient eukaryotic protein expression system, which can successfully achieve intracellular expression or secretory expression, and its amplification medium is relatively cheap, and the culture conditions are not demanding, so it is suitable for industrial amplification. Like mammalian cell expression systems, yeast protein expression systems can performRead more
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Oct 28,2021
Yeast Expression System and Method
The Production of Yeast Expression System The development of genetic engineering technology has shown broad prospects for the synthesis and production of foreign proteins by microorganisms. For a long time, people have used E. coli as a host to express a variety of proteins. This is because E. coli has several advantages, such as clearRead more
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Oct 26,2021
Common Protein Expression System
Introduction In vitro recombinant protein production is a protein obtained by applying recombinant DNA or recombinant RNA technology. Catalog The use of in vitro protein expression technology to extensively produce various types of recombinant proteins, which are used in the fields of medicine, food, daily chemical and other fields. The following briefly introduces the mainRead more
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Oct 26,2021
Application of Baculovirus-insect Cell Expression System
Introduction to Bac-to-Bac System The baculovirus-insect cell system is a binary system consisting of two parts. The first part is the baculovirus expression vector, which is an insect virus whose function is obviously to introduce the foreign gene encoding the target protein into the host cell. Another function of baculovirus expression vectors is to provideRead more
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Oct 26,2021
Comparison of E. coli and Insect Baculovirus Expression Systems
Principle of Escherichia coli Expression System The Escherichia coli expression system is an expression system that efficiently expresses genes encoding protective antigens of pathogenic microorganisms in Escherichia coli through DNA recombination technology. It is currently the most commonly used system for recombinant protein expression. The E. coli expression system has been studied earlier and hasRead more
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Oct 25,2021
Baculovirus Expression Vector System and its Application
The most researched insect baculovirus is Autographa californica multiple nuclear polyhedrosisvirus (AcMNPV), its host is Spodoptera frugiperda (Sf); Bombyx mori nuclear polyhedrosis virus (Bombyx) mori nuclear polyhedrosis virus (BmNPV), whose host is the silkworm (Bombyx mori), these two baculovirus vectors are often used to express foreign proteins [2,3]. The gene expression of baculovirus is dividedRead more
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Oct 25,2021
What is Baculovirus Expression System and its Advantages
What is Baculovirus Expression System Baculovirus belongs to the Baculoviridae family (Baculoviridae), which is divided into nuclear polyhedrosis virus and granulovirus. The virus particles are rod-shaped and are a type of DNA virus that specifically infects arthropods and has an envelope. Genomic DNA is compressed and packed in a rod-shaped capsid in a super-coiled form,Read more
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Sep 23,2021
Common ELISA Format
The competitive ELISA format is based on the binding competition in the initial insulin RIA and IgG EIA methods. The competition between the target analyte from the sample and the reference analyte and the binding of the analyte-specific antibody is the key to this format. Either antibody or target analyte can be labeled with enzymeRead more
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Sep 03,2021
Things you have to know about the Western Blot Experiment
Western Blot, also known as western blot analysis and immunoblotting, is a technique widely used in the detection and analysis of proteins. It uses polyacrylamide gel electrophoresis. “Use a labeled secondary antibody. Western blot analysis is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. Medicilon Biopharmaceuticals can provide Western blotting experimental technologyRead more
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Sep 03,2021
Application of Western Blot Analysis in Studying the Proliferation of NM23B Gene Cells
NM23 is a tumor metastasis suppressor gene, which has regulatory functions on cell proliferation, differentiation and ontogeny. At present, 10 subtypes of NM23 have been found, and the more thorough research is NM23A and NM23B. Western blot analysis shows that NM23B has hepatocyte proliferation effect on SD rats cultured in vitro. Western blot analysis, alsoRead more
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Sep 03,2021
Western blot analysis technique in detecting protein expression level
Lung cancer is one of the most harmful malignant tumors. To explore the pathogenesis of lung cancer and find new targets for anti-tumor therapy are the hotspots and difficulties in the current research on lung cancer treatment. Studies have found that aquaporin 4 (AQP4) is highly expressed in lung cancer cells, and knocking down AQP4Read more
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Aug 31,2021
Application of Yeast Two-hybrid Technology in Screening Drugs and Finding Drug Targets
The yeast two-hybrid system is an important technical means for studying protein-protein interactions, and is now also being used in high-throughput screening of interacting proteins and other related molecules. Yeast transformation is simple and efficient. After the yeast two-hybrid library is constructed, it is relatively easy to directly screen out positive clones that interact betweenRead more
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Aug 31,2021
Yeast two-hybrid technology can be used to screen LKB1 interacting proteins
It is well known that proteins play a very key role in the process of cell life activities. The understanding of protein interactions can better understand the essence of life. The yeast two-hybrid system is an effective genetic method for analyzing protein interactions in vivo. In the yeast two-hybrid experiment service provided by some biomedicalRead more
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Aug 24,2021
Potassium ion channel, calcium ion channel, sodium ion channel
Ion channels are channels on the cell membrane that can regulate and transport specific ions through the cell membrane. They are usually composed of protein molecules, and often several transmembrane protein subunits form an ion channel. It is an important basis for maintaining the information transmission of the body’s nervous system, the rhythmic beating ofRead more
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Aug 19,2021
How to do a good job of biological analysis of peptide drugs?
Since Robert Bruce Merrifield synthesized the first biologically active peptide in 1953, research on peptide drugs has developed rapidly, and research on peptide drugs targeting multiple receptors has even been carried out internationally. The maturity of peptide synthesis technology has further accelerated the development of peptide drugs and has become one of the international researchRead more
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Aug 19,2021
Experimental Steps of Western Blotting Test
Western blot is a protein detection and analysis technique widely used in the fields of biology and medicine. It can be used to detect the expression level of specific proteins in tissue homogenates or cell extracts. Western blotting technology steps are mainly divided into two parts, namely SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The BiologyRead more
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Aug 18,2021
Western Blot Detection
The Biology Department of Shanghai Medicilon has extensive experience in the field of in vitro biology, through enzyme level determination, cell level determination, cell biology, biochemistry, in vitro isotope determination, stable cell line establishment, gene knockout, RNAi and MicroRNA Technology, etc., to provide a complete set of biological services. In cell biology research, sometimes itRead more
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Aug 10,2021
Protein Purification Methods and Comparison of Advantages and Disadvantages
The separation and purification of proteins is more complicated. Proteins extracted from cells or proteins obtained from solutions containing proteins through precipitation, gradient centrifugation, salting out and other methods often contain impurities. These impurities must be removed while maintaining the biological characteristics of the protein. For the chemical activity, such as the catalytic activity ofRead more
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