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- Flow Cytometry is a modern multi-parameter cell analysis technology that can detect single particles at high speed, accurately and objectively. At present, in the early stage of drug development, especially for anti-tumor drugs, complex cell analysis through flow multicolor detection technology can provide scientific researchers with maximum effective data.
- Up to now, Medicilon has undertaken nearly 400 flow cytometry detection projects, including：Detection of cell surface antigen expression; Analysis of cell proliferation/ differentiation/apoptosis; Detection of animal PBMCs/ immune organs; Analysis of xenograft model.Medicilon flow cytometry detection team combines the characteristics of each case with years of practical experience and technical accumulation. They deliver high-quality experimental results to clients to help customers' R&D projects get approved.
Application of Flow Cytometry- Flow cytometry technology has obvious advantages in the early research of anti-tumor drugs:In the innovative research of combination of therapeutic targets and drugs, it could help researchers to show the differences in biological effects between different individuals in the preliminary work of drug development, so as to further clarify the mechanism of action; To help researchers perform high-throughput and robust quantitative analysis of the tumor’s “microenvironment”, peripheral blood and the immune system in other host tissues, to achieve more clearly predicting the efficacy of immunotherapy, and to help the large-scale immunotherapy methods development.Flow cytometry has been utilized at each stage of the drug discovery process, from target identification to lead development. Valid drug targets in cells encompass almost the entire plethora of biomolecular structures available: the cell membrane, any protein involved in mechanistic function or signaling.
- Characterization of large and varied cell populations in a high throughput manner is of use in initial target identification, allowing dysfunctional cells to be separated for further analysis by other techniques. This process can be optimized by tagging molecular features with the cell suspected to be involved in disease with fluorescent probes, allowing structural features of the cell such as the abundance of a particular protein or the integrity of the cell membrane can be quickly assessed. Following target identification and the generation of a drug lead, efficacy and toxicity of the lead can be investigated by flow cytometry.
- Flow Cytometry in ImmunologyFlow cytometry has been conventionally associated with the use of monoclonal antibodies to identify immuno-competent cells, to quantify changes in expression of surface determinants, and to separate cell population subsets before testing their functional characteristics.Multi-laser system, developments of multicolor fluorescence, and improvements of computing systems for multi-parametric analysis helps in the improvement of diagnosis, prognosis, and monitoring therapies given. Advances in flow cytometry now allow for automated, multiparametric analyses of hundreds of samples in a day.
- Advantages of Flow Cytometry TechnologyIn assessing combination of therapeutic targets and drugs, flow cytometry can help researchers to show differences in biological effects between different individuals to further clarify the mechanism of effectiveness; Flow cytometry can be used to help researchers perform high-throughput and quantitative analysis of the tumor’s “microenvironment”, peripheral blood and the immune system in other host tissues, to more clearly predicting the efficacy of immunotherapy and to help the large-scale immunotherapy methods development.
Medicilon Flow Cytometry Technology Platform- Medicilon’s streaming technology platform is committed to exploring and insight into the progress of tumor immune targeted therapy drugs and providing accurate and reliable test prediction data in the early development stage. Our team has rich experience in experimental operation, streaming method development and data analysis . The platform is equipped with 4 powerful flow BD cytometers. The newly introduced high-parameter BD FACSymphony A5 flow cytometer is equipped with five lasers and 31 parameters, which can enable up to 29 colors at the same time to meet clients’ scientific research and analysis needs.
- Development Methodolgy and Sample TestingMedicilon’s flow cytometry team has experience in the development of flow cytometry methods for mouse, rat, dog, monkey, human and other species, as well as animal models of immunodeficiency, genetic modification and humanization. Medicilon also has experience in processing and analysing various samples including peripheral blood, spleen, lymph node, bone marrow, tumor, liver and lung.
- Established a basic analysis program (T, B, NK cells) for a complex detection analysis program (regulatory T cells, myeloid cell analysis, stem cell differentiation, etc.), involving cell surface proteins, intracellular/nuclear proteins detection (Transcription factors / Chemokines / Cytokines), to meet the needs of clients for detection and analysis.
- Medicilon’s flow platform provides cytokine detection services using flow CBA, Bio-Plex 200 suspension chip technology. Only a small amount of sample is required for each test,# to deliver excellent test specificity, good stability and high repeatability.
- Antibody Drug Receptor Occupancy Rate (RO) ProjectSince 2017, a number of clinical/preclinical RO tests have been completed, involving the detection of many different targets such as PD-1, CD20, CD47 and TIGIT.
- Biological Analysis of New Immune Cell DrugsIncluding stem cells, CAR-T cells and CAR-NK cells and other cell therapy products.
Assay Cell Medicilon capability
(Human/Mouse/Rat/Monkey/Dog)FACS T cell /Treg Yes (Human/Mouse/Rat/Monkey/Dog) B cell Yes (Human/Mouse/Rat/Monkey/Dog) NK cell Yes (Human/Mouse/Rat/Monkey) Macrophage / Monocyte / Neutrophil Yes (Human/Mouse/Monkey) DC cell Yes (Human/Mouse/Monkey) MDSC Yes (Human/Mouse/Monkey) Checkpoint Yes (Human/Mouse) Receptor Occupancy Yes (Human/Mouse/Monkey) Cytokine Phosphorylation Yes (Human/Mouse) Elisa Yes (Human/Mouse/Rat/Monkey) CBA Yes (Human/Mouse/Rat/Monkey) Luminex Yes (Human/Mouse/Rat/Monkey) MSD Yes (Human/Mouse/Rat/Monkey) ELISpot / FluoroSpot Yes (Human/Mouse/Monkey) Cell differentiation/polarization T/M1/M2 macrophage Yes (Human) Cell cytotoxicity ADCC, ADCP Yes (Human) To be continue
Examples of flow cytometric detection of cynomolgus monkey cell subpopulations
Examples of flow cytometric detection of mouse NK and T cell subsets - Medicilon FACS Assay EquipmentDifferent blood and tissue samples can be detected by BD Symphony A3/Fortessa/Celesta for 50+ Validated Panels (Up to 29 markers)
With 5 lasers, 29 channels configuration
Utilize the total reflection optical path structure design to avoid the loss of optical signal in the transmission process, improve the sensitivity of analysis, and be able to identify and analyze rare cell types and conditions.
An advanced streaming product,which could acquire and analyze the big data of the immune system and provide a platform for exploring unknown immunoomics.
- Medicilon Case: FACS AssayBlood/Lymph Node/Spleen/Bone Marrow/Tumor… samples can be analysed with FLOWJO for 50+ Validated Panels after FCM processed and detected.
Data shown was Mouse Treg&Teff Panel_mouse_TumorBlood/Lymph Node/Spleen/Bone Marrow/Tumor… samples could be analysed with FLOWJO for 50+ Validated Panels after FCM processed and detected.
Data shown was Cyno T/B/NK Panel_monkey_BloodBlood samples could be analysed with FLOWJO after FCM processed and detected.
Data shown was Receptor Occupancy_Cynomolgus monkey_Blood - Medicilon Cytokine Analysis EquipmentDifferent serum/plasma and tissue samples can be analysed and detected by Covaris E220R/BIO-RAD Bio-Plex 200/MD SPECTRAMAX M2/MSD for cytokine panels (up to 40+ factors)
- Medicilon Case: Cytokine AnalysisSerum/Plasma/Liver/Lung/Tumor … samples can be analysed after processed and detected.
Data shown was CBA_Cynomolgus monkey_PlamsaPBMC/MNC samples can be analysed by CTL.
Data shown was EliSpot/FluoroSpot_PBMC - Cell Cytotoxicity AssayCell cytotoxicity can be detected with a flow cytometer, microplate reader or fluorescence microscope;We can detects cell differentiation and cytolytic activity with multiple types of mammalian cell lines.
- Medicilon Case: Cell Cytotoxicity AssayCell line/Blood/Tissue samples can be analysed with FLOWJO.
Data shown was ADCP_PBMC
Future Applications- Flow cytometry can be used to study vaccine efficacy and safety. Modern high-end flow cytometers are capable of processing tens of thousands of cells per second in an entirely automated manner, though when undertaking higher degrees of characterization analysis is slower. Continuously running bioreactors generate cells for testing that can then be exposed to a variety of conditions and later categorized by flow cytometry. Increased automation, cell processing speed, and a variety of characterization techniques incorporated directly into flow cytometry equipment will be developed by Medicilon going forward.
