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Email:marketing@medicilon.com
+1(626)986-9880(U.S.)
0044 7790 816 954 (Europe)
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Email: marketing@medicilon.com.cn
Tel: +86 (21) 5859-1500
ELISA is a biochemical or biological technique used to detect the presence of certain components in a sample. Depending on the type of ELISA, it can be used to detect either an antibody (for example, in diagnosing HIV, it tests for the presence of antibodies to the virus), or a target compound (antigen), for example PAPP-A. While this technique is commonly used in immunology, developments in antibody production allow its use in the detection and analysis of a wide range of chemical compounds. It is often the method of choice for detecting biological molecules such as proteins.
ELISA is a test that can be used to detect either antibody (Ab) or antigen such as viral proteins. There are four kinds of ELISA assay tests: Direct ELISA, Indirect ELISA, Sandwich ELISA and Competitive ELISA.
Generally, an ELISA has the following steps (each separated by extensive washes):
Antigen Binding: A solid support surface is initially coated with an appropriate amount of the antigen of interest. The whole surface must be blocked to ensure biologically specific binding of the subsequent components. Having the antigen bound to the solid surfaced makes it simple to separate bound from unbound molecules. Non specifically bound materials are washed away during the process of the assay.
Blocking: All unbound sites on the solid support are blocked to prevent nonspecific binding of the antibodies.
Primary Antibody: The primary antibody is added and will be bound if there is a recognized epitope within sample antigen.
Secondary Antibody: An enzyme-linked secondary antibody is added which will bind to any available primary antibody. Primary antibody will only be available on the surface if it has bound to the antigen.
Detection: After adding an enzyme substrate, a colored product will develop if this binding has occurred. The color intensity reflects the amount of primary antibody bound to the target antigen and can be measured.
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Tips: Above is part of Elisa assay services and Elisa test steps. You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.