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Introduction to indirect immunofluorescence

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What is immunofluorescence technology?

Immunofluorescence technology is an immunoassay technique that combines the specificity of antigen-antibody reaction with the sensitivity and intuitiveness of fluorescent substance detection.

What are the types of immunofluorescence technology?

  1. Fluorescent antibody staining technology: fluorescence microscopy technology, flow cytometry immunofluorescence technology

  2. (2) Fluorescence immunoassay: time-resolved immunoassay, fluorescence polarization immunoassay

What are the commonly used fluorescent substances?

Fluorescein isothiocyanate(FITC), tetraethyl rhodamine (RB200), tetramethyl rhodamine isothiocyanate, phycoerythrin, Lan family rare earth elements, etc.

What are the fluorescence immunomicroscopy techniques?

Direct method, indirect method, complement fixation method, double labeling method.

What is indirect immunofluorescence?

After using the corresponding antigen in the matrix to react with the antibody (primary antibody) in the sample to be tested, fluorescein-labeled anti-antibody (second antibody) is combined with the primary antibody, and then the antigen or antibody is checked by fluorescence. This method is often used to detect autoantibodies (such as ANA).

Positive fluorescence spectrum

Let’s take ANA as an example, and attach several common ANA fluorescence pictures in the laboratory for everyone to enjoy.

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